Directed evolution: methodologies and applications
Directed evolution aims to expedite the natural evolution process of biological molecules
and systems in a test tube through iterative rounds of gene diversifications and library …
and systems in a test tube through iterative rounds of gene diversifications and library …
Fluorescent proteins and genetically encoded biosensors
M Wang, Y Da, Y Tian - Chemical Society Reviews, 2023 - pubs.rsc.org
The genetically encoded fluorescent sensors convert chemical and physical signals into
light. They are powerful tools for the visualisation of physiological processes in living cells …
light. They are powerful tools for the visualisation of physiological processes in living cells …
A highly photostable and bright green fluorescent protein
M Hirano, R Ando, S Shimozono, M Sugiyama… - Nature …, 2022 - nature.com
The low photostability of fluorescent proteins is a limiting factor in many applications of
fluorescence microscopy. Here we present StayGold, a green fluorescent protein (GFP) …
fluorescence microscopy. Here we present StayGold, a green fluorescent protein (GFP) …
Morphogenic Regulators Baby boom and Wuschel Improve Monocot Transformation
K Lowe, E Wu, N Wang, G Hoerster, C Hastings… - The Plant …, 2016 - academic.oup.com
While transformation of the major monocot crops is currently possible, the process typically
remains confined to one or two genotypes per species, often with poor agronomics, and …
remains confined to one or two genotypes per species, often with poor agronomics, and …
A guide to fluorescent protein FRET pairs
Förster or fluorescence resonance energy transfer (FRET) technology and genetically
encoded FRET biosensors provide a powerful tool for visualizing signaling molecules in live …
encoded FRET biosensors provide a powerful tool for visualizing signaling molecules in live …
Hybrid small-molecule/protein fluorescent probes
M Minoshima, SI Reja, R Hashimoto, K Iijima… - Chemical …, 2024 - ACS Publications
Hybrid small-molecule/protein fluorescent probes are powerful tools for visualizing protein
localization and function in living cells. These hybrid probes are constructed by diverse site …
localization and function in living cells. These hybrid probes are constructed by diverse site …
Microbial whole-cell biosensors: Current applications, challenges, and future perspectives
Abstract Microbial Whole-Cell Biosensors (MWCBs) have seen rapid development with the
arrival of 21st century biological and technological capabilities. They consist of microbial …
arrival of 21st century biological and technological capabilities. They consist of microbial …
Fluorescent proteins and their applications in imaging living cells and tissues
Green fluorescent protein (GFP) from the jellyfish Aequorea victoria and its homologs from
diverse marine animals are widely used as universal genetically encoded fluorescent labels …
diverse marine animals are widely used as universal genetically encoded fluorescent labels …
Green fluorescent protein as a marker for gene expression
M Chalfie, Y Tu, G Euskirchen, WW Ward, DC Prasher - Science, 1994 - science.org
A complementary DNA for the Aequorea victoria green fluorescent protein (GFP) produces a
fluorescent product when expressed in prokaryotic (Escherichia coli) or eukaryotic …
fluorescent product when expressed in prokaryotic (Escherichia coli) or eukaryotic …
Quantum dot bioconjugates for imaging, labelling and sensing
IL Medintz, HT Uyeda, ER Goldman, H Mattoussi - Nature materials, 2005 - nature.com
One of the fastest moving and most exciting interfaces of nanotechnology is the use of
quantum dots (QDs) in biology. The unique optical properties of QDs make them appealing …
quantum dots (QDs) in biology. The unique optical properties of QDs make them appealing …