Directed evolution: methodologies and applications

Y Wang, P Xue, M Cao, T Yu, ST Lane… - Chemical reviews, 2021 - ACS Publications
Directed evolution aims to expedite the natural evolution process of biological molecules
and systems in a test tube through iterative rounds of gene diversifications and library …

Fluorescent proteins and genetically encoded biosensors

M Wang, Y Da, Y Tian - Chemical Society Reviews, 2023 - pubs.rsc.org
The genetically encoded fluorescent sensors convert chemical and physical signals into
light. They are powerful tools for the visualisation of physiological processes in living cells …

A highly photostable and bright green fluorescent protein

M Hirano, R Ando, S Shimozono, M Sugiyama… - Nature …, 2022 - nature.com
The low photostability of fluorescent proteins is a limiting factor in many applications of
fluorescence microscopy. Here we present StayGold, a green fluorescent protein (GFP) …

Morphogenic Regulators Baby boom and Wuschel Improve Monocot Transformation

K Lowe, E Wu, N Wang, G Hoerster, C Hastings… - The Plant …, 2016 - academic.oup.com
While transformation of the major monocot crops is currently possible, the process typically
remains confined to one or two genotypes per species, often with poor agronomics, and …

A guide to fluorescent protein FRET pairs

BT Bajar, ES Wang, S Zhang, MZ Lin, J Chu - Sensors, 2016 - mdpi.com
Förster or fluorescence resonance energy transfer (FRET) technology and genetically
encoded FRET biosensors provide a powerful tool for visualizing signaling molecules in live …

Hybrid small-molecule/protein fluorescent probes

M Minoshima, SI Reja, R Hashimoto, K Iijima… - Chemical …, 2024 - ACS Publications
Hybrid small-molecule/protein fluorescent probes are powerful tools for visualizing protein
localization and function in living cells. These hybrid probes are constructed by diverse site …

Microbial whole-cell biosensors: Current applications, challenges, and future perspectives

M Moraskie, MHO Roshid, G O'Connor, E Dikici… - Biosensors and …, 2021 - Elsevier
Abstract Microbial Whole-Cell Biosensors (MWCBs) have seen rapid development with the
arrival of 21st century biological and technological capabilities. They consist of microbial …

Fluorescent proteins and their applications in imaging living cells and tissues

DM Chudakov, MV Matz, S Lukyanov… - Physiological …, 2010 - journals.physiology.org
Green fluorescent protein (GFP) from the jellyfish Aequorea victoria and its homologs from
diverse marine animals are widely used as universal genetically encoded fluorescent labels …

Green fluorescent protein as a marker for gene expression

M Chalfie, Y Tu, G Euskirchen, WW Ward, DC Prasher - Science, 1994 - science.org
A complementary DNA for the Aequorea victoria green fluorescent protein (GFP) produces a
fluorescent product when expressed in prokaryotic (Escherichia coli) or eukaryotic …

Quantum dot bioconjugates for imaging, labelling and sensing

IL Medintz, HT Uyeda, ER Goldman, H Mattoussi - Nature materials, 2005 - nature.com
One of the fastest moving and most exciting interfaces of nanotechnology is the use of
quantum dots (QDs) in biology. The unique optical properties of QDs make them appealing …