Smart chemistry and applied perceptions of enzyme-coupled nano-engineered assemblies to meet future biocatalytic challenges

A Anwar, M Imran, HMN Iqbal - Coordination Chemistry Reviews, 2023 - Elsevier
Enzymes have received extensive research due to their high selectivity, efficiency, and
environmental protection. However, the lack of stability and reusability drastically hinders the …

3D printing and enzyme immobilization: An overview of current trends

D Remonatto, BF Izidoro, VT Mazziero, BP Catarino… - Bioprinting, 2023 - Elsevier
Given their enormous flexibility and freedom of design, 3D printing technologies have been
applied in various fields, such in the production of high value-added products via …

Gene expression and molecular characterization of recombinant subtilisin from Bacillus subtilis with antibacterial, antioxidant and anticancer properties

SS Shettar, ZK Bagewadi, DA Yaraguppi, S Das… - International Journal of …, 2023 - Elsevier
This study investigated the multifunctional attributes such as, antibacterial, antioxidant and
anticancer potential of recombinant subtilisin. A codon-optimized subtilisin gene was …

Surface activation of hierarchically porous diatomite modified with polyethyleneimine for immobilizing d-allulose 3-epimerase

Z Xiao, Z Zhao, B Jiang, J Chen - Food Bioscience, 2023 - Elsevier
Abstract d-Allulose, a low-calorie functional sweetener popular among the obese
population, can be biosynthesized using d-allulose 3-epimerase (DAE). However, there are …

Enhancing enzyme immobilization: Fabrication of biosilica-based organic-inorganic composite carriers for efficient covalent binding of D-allulose 3-epimerase

Z Xiao, Z Zhao, B Jiang, J Chen - International Journal of Biological …, 2024 - Elsevier
D-allulose, an ideal low-calorie sweetener, is primarily produced through the isomerization
of d-fructose using D-allulose 3-epimerase (DAE; EC 5.1. 3.30). Addressing the gap in …

Combined cross-linked enzyme aggregates of cyclodextrin glucanotransferase and maltogenic amylase from Bacillus lehensis G1 for maltooligosaccharides synthesis

YS Yip, NHA Manas, NR Jaafar, RA Rahman… - International Journal of …, 2023 - Elsevier
Maltooligosaccharides (MOS) are functional oligosaccharides that can be synthesized
through enzymatic cascade reaction between cyclodextrin glucanotransferase (CGTase) …

Covalent bonding immobilization of a Bacillus licheniformis protease on chitosan and its application in protein hydrolysis

EX Ramalho, RJS De Castro - Biocatalysis and Agricultural Biotechnology, 2023 - Elsevier
Enzyme immobilization makes its structure more rigid and insoluble, increasing its stability.
This can be done by forming a covalent bond between the enzyme and an inert, insoluble …

Enhancement of β-galactosidase catalytic activity and stability through covalent immobilization onto alginate/tea waste beads and evaluating its impact on the quality …

ME Hassan, GE Ibrahim, MAA Abdella - International Journal of Biological …, 2024 - Elsevier
The current study aimed to evaluate the hydrolysis of whole fat milk (WFM) and sweet whey
(SW) using β-galactosidase (β-gal) after covalent immobilization onto activated alginate/tea …

Detection of insecticides by Tetronarce californica acetylcholinesterase via expression and in silico analysis

S Jiang, Q Gu, X Yu - Applied Microbiology and Biotechnology, 2023 - Springer
The acetylcholinesterase (AChE) is involved in termination of synaptic transmission at
cholinergic synapses and plays a vital role in the insecticide detection and inhibitor …

[HTML][HTML] Biochemical characterization of immobilized recombinant subtilisin and synthesis and functional characterization of recombinant subtilisin capped silver and …

SS Shettar, ZK Bagewadi, TMY Khan… - Saudi Journal of …, 2024 - Elsevier
This pioneering research explores the transformative potential of recombinant subtilisin,
emphasizing its strategic immobilization and nanoparticle synthesis to elevate both stability …