Sheep and goat genome engineering: from random transgenesis to the CRISPR era

P Kalds, S Zhou, B Cai, J Liu, Y Wang… - Frontiers in …, 2019 - frontiersin.org
Sheep and goats are valuable livestock species that have been raised for their production of
meat, milk, fiber, and other by-products. Due to their suitable size, short gestation period …

Current status of assisted reproductive technologies in buffaloes

K Srirattana, D Hufana‐Duran, EP Atabay… - Animal Science …, 2022 - Wiley Online Library
Buffaloes are raised by small farm holders primarily as source of draft power owing to its
resistance to hot climate, disease, and stress conditions. Over the years, transformation of …

Handmade cloning: recent advances, potential and pitfalls

G Verma, JS Arora, RS Sethi… - Journal of animal …, 2015 - Springer
Handmade cloning (HMC) is the most awaited, simple and micromanipulator-free version of
somatic cell nuclear transfer (SCNT). The requirement of expensive micromanipulators and …

Effect of post-fusion holding time, orientation and position of somatic cell-cytoplasts during electrofusion on the development of handmade cloned embryos in buffalo …

NL Selokar, RA Shah, AP Saha, M Muzaffar, M Saini… - Theriogenology, 2012 - Elsevier
The present study was conducted primarily to optimize electrofusion conditions for efficient
production of zona-free nuclear transfer embryos in buffalos (Bubalus bubalis). We found …

Cloning of buffalo, a highly valued livestock species of south and southeast Asia: any achievements?

NL Selokar, M Saini, P Palta, MS Chauhan… - Cellular …, 2018 - liebertpub.com
Buffalo (Bubalus bubalis) is a major source of milk, meat, and draught power in many
developing countries in Asia. Animal cloning holds a lot of potential for fast multiplication of …

Roscovitine Treatment Improves Synchronization of Donor Cell Cycle in G0/G1 Stage and In Vitro Development of Handmade Cloned Buffalo (Bubalus bubalis) …

NL Selokar, M Saini, M Muzaffer… - … " Cloning and Stem …, 2012 - liebertpub.com
This study investigated the effects of serum-starvation, total confluence, and roscovitine
treatment on cell-cycle synchronization of buffalo ear skin fibroblasts to the G0/G1stage and …

Effect of donor cell type on developmental competence, quality, gene expression, and epigenetic status of interspecies cloned embryos produced using cells from wild …

M Saini, NL Selokar, AK Raja, AA Sahare, SK Singla… - Theriogenology, 2015 - Elsevier
This study compared the cloning efficiency of donor cells of fibroblast and epithelial origin
isolated from ear skin of a wild buffalo (Bubalus arnee) and used with cytoplasts from …

Handmade cloned and parthenogenetic goat embryos–A comparison of different culture media and donor cells

MK Jena, D Malakar, AK De, S Garg, YS Akshey… - Small ruminant …, 2012 - Elsevier
The aim of the present investigation was the production of handmade cloned and
parthenogenetic goat embryos. One somatic cell was attached to an enucleated demi …

Production of Wild Buffalo (Bubalus arnee) Embryos by Interspecies Somatic Cell Nuclear Transfer Using Domestic Buffalo (Bubalus bubalis) Oocytes

D Priya, NL Selokar, AK Raja, M Saini… - … in Domestic Animals, 2014 - Wiley Online Library
Contents The objective of this study was to explore the possibility of producing wild buffalo
embryos by interspecies somatic cell nuclear transfer (iSCNT) through handmade cloning …

Cryopreservation of Zona‐Free Cloned Buffalo (Bubalus Bubalis) Embryos: Slow Freezing vs Open‐Pulled Straw Vitrification

K Sirisha, NL Selokar, M Saini, P Palta… - … in Domestic Animals, 2013 - Wiley Online Library
Contents This study was carried out to compare the post‐thaw cryosurvival rate and the level
of apoptosis in vitro produced zona‐free cloned buffalo blastocysts subjected to slow …