Multiplexed CRISPR technologies, in which numerous gRNAs or Cas enzymes are
expressed at once, have facilitated powerful biological engineering applications, vastly …

Metabolic engineering aims to develop efficient cell factories by rewiring cellular
metabolism. As one of the most commonly used cell factories, Saccharomyces cerevisiae …

The tetrahydroisoquinoline (THIQ) moiety is a privileged substructure of many bioactive
natural products and semi-synthetic analogs. Plants manufacture more than 3,000 THIQ …

Designing an optimal microbial cell factory often requires overexpression, knock-down, and
knock-out of multiple gene targets. Unfortunately, such rewiring of cellular metabolism is …

Despite the extensive use of Saccharomyces cerevisiae as a platform for synthetic biology,
strain engineering remains slow and laborious. Here, we employ CRISPR/Cas9 technology …

The term 'clustered regularly interspaced short palindromic repeats'(CRISPR) has recently
become synonymous with the genome-editing revolution. The RNA-guided endonuclease …

Genome editing with CRISPR-Cas nucleases has been applied successfully to a wide range
of cells and organisms. There is, however, considerable variation in the efficiency of …

The CRISPR-Cas9 bacterial surveillance system has become a versatile tool for genome
editing and gene regulation in eukaryotic cells, yet how CRISPR-Cas9 contends with the …

The methods based on the clustered regularly interspaced short palindromic repeats
(CRISPR)/CRISPR-associated (Cas) system have quickly gained popularity for genome …

Within five years, the CRISPR-Cas system has emerged as the dominating tool for genome
engineering, while also changing the speed and efficiency of metabolic engineering in …