Conformational heterogeneity and probability distributions from single-particle cryo-electron microscopy
Single-particle cryo-electron microscopy (cryo-EM) is a technique that takes projection
images of biomolecules frozen at cryogenic temperatures. A major advantage of this …
images of biomolecules frozen at cryogenic temperatures. A major advantage of this …
Methods for cryo-EM single particle reconstruction of macromolecules having continuous heterogeneity
B Toader, FJ Sigworth, RR Lederman - Journal of molecular biology, 2023 - Elsevier
Macromolecules change their shape (conformation) in the process of carrying out their
functions. The imaging by cryo-electron microscopy of rapidly-frozen, individual copies of …
functions. The imaging by cryo-electron microscopy of rapidly-frozen, individual copies of …
Integrating molecular models into CryoEM heterogeneity analysis using scalable high-resolution deep Gaussian mixture models
Resolving the structural variability of proteins is often key to understanding the structure–
function relationship of those macromolecular machines. Single particle analysis using …
function relationship of those macromolecular machines. Single particle analysis using …
Computational biophysics meets cryo‐EM revolution in the search for the functional dynamics of biomolecular systems
There is a variety of experimental and computational techniques available to explore protein
dynamics, each presenting advantages and limitations. One promising experimental …
dynamics, each presenting advantages and limitations. One promising experimental …
Towards interpretable Cryo-EM: disentangling latent spaces of molecular conformations
Molecules are essential building blocks of life and their different conformations (ie, shapes)
crucially determine the functional role that they play in living organisms. Cryogenic Electron …
crucially determine the functional role that they play in living organisms. Cryogenic Electron …
CryoSTAR: leveraging structural priors and constraints for cryo-EM heterogeneous reconstruction
Resolving conformational heterogeneity in cryogenic electron microscopy datasets remains
an important challenge in structural biology. Previous methods have often been restricted to …
an important challenge in structural biology. Previous methods have often been restricted to …
Revealing biomolecular structure and motion with neural ab initio cryo-EM reconstruction
Proteins and other biomolecules form dynamic macromolecular machines that are tightly
orchestrated to move, bind, and perform chemistry. Cryo-electron microscopy (cryo-EM) can …
orchestrated to move, bind, and perform chemistry. Cryo-electron microscopy (cryo-EM) can …
Reconstructing Heterogeneous Cryo-EM Molecular Structures by Decomposing Them into Polymer Chains
Cryogenic electron microscopy (cryo-EM) has transformed structural biology by allowing to
reconstruct 3D biomolecular structures up to near-atomic resolution. However, the 3D …
reconstruct 3D biomolecular structures up to near-atomic resolution. However, the 3D …
[PDF][PDF] Digital Twins for Enhanced Modeling and Control of Light Sources and Neutron Sources
Light sources and neutron sources play a key role in understanding the fundamental
properties of complex matter at different time and length scales by capturing their structural …
properties of complex matter at different time and length scales by capturing their structural …
Physics-informed geometric regularization of heterogeneous reconstructions in cryo-EM
Many proteins are flexible and occur in a continuum of 3D conformations. A protein's 3D
conformation is the main determinant of its biological function, and is therefore of paramount …
conformation is the main determinant of its biological function, and is therefore of paramount …