A roadmap for metagenomic enzyme discovery

SL Robinson, J Piel, S Sunagawa - Natural Product Reports, 2021 - pubs.rsc.org
Covering: up to 2021 Metagenomics has yielded massive amounts of sequencing data
offering a glimpse into the biosynthetic potential of the uncultivated microbial majority. While …

Microbial production of limonene and its derivatives: Achievements and perspectives

Y Ren, S Liu, G Jin, X Yang, YJ Zhou - Biotechnology Advances, 2020 - Elsevier
Limonene and its derivatives have great market potential with diverse applications in food,
pharmaceuticals, cosmetics, etc. Commercial production of limonene and its derivatives …

Cell-free prototyping enables implementation of optimized reverse β-oxidation pathways in heterotrophic and autotrophic bacteria

B Vögeli, L Schulz, S Garg, K Tarasava… - Nature …, 2022 - nature.com
Carbon-negative synthesis of biochemical products has the potential to mitigate global CO2
emissions. An attractive route to do this is the reverse β-oxidation (r-BOX) pathway coupled …

In vitro prototyping of limonene biosynthesis using cell-free protein synthesis

QM Dudley, AS Karim, CJ Nash, MC Jewett - Metabolic Engineering, 2020 - Elsevier
Metabolic engineering of microorganisms to produce sustainable chemicals has emerged
as an important part of the global bioeconomy. Unfortunately, efforts to design and engineer …

Acoustic ejection mass spectrometry for high-throughput analysis

H Zhang, C Liu, W Hua, LP Ghislain, J Liu… - Analytical …, 2021 - ACS Publications
We describe a mass spectrometry (MS) analytical platform resulting from the novel
integration of acoustic droplet ejection (ADE) technology, an open-port interface (OPI), and …

Total in vitro biosynthesis of the nonribosomal macrolactone peptide valinomycin

L Zhuang, S Huang, WQ Liu, AS Karim, MC Jewett… - Metabolic …, 2020 - Elsevier
Natural products are important because of their significant pharmaceutical properties such
as antiviral, antimicrobial, and anticancer activity. Recent breakthroughs in DNA sequencing …

Enabling pathway design by multiplex experimentation and machine learning

AG Boob, J Chen, H Zhao - Metabolic Engineering, 2024 - Elsevier
The remarkable metabolic diversity observed in nature has provided a foundation for
sustainable production of a wide array of valuable molecules. However, transferring the …

Cell-free styrene biosynthesis at high titers

WS Grubbe, BJ Rasor, A Krüger, MC Jewett… - Metabolic …, 2020 - Elsevier
Styrene is an important petroleum-derived molecule that is polymerized to make versatile
plastics, including disposable silverware and foamed packaging materials. Finding more …

Biological materials: the next frontier for cell-free synthetic biology

RJR Kelwick, AJ Webb, PS Freemont - Frontiers in Bioengineering …, 2020 - frontiersin.org
Advancements in cell-free synthetic biology are enabling innovations in sustainable
biomanufacturing, that may ultimately shift the global manufacturing paradigm toward …

Acoustic ejection mass spectrometry: fundamentals and applications in high-throughput drug discovery

C Liu - Expert Opinion on Drug Discovery, 2022 - Taylor & Francis
Introduction Acoustic ejection mass spectrometry (AEMS) is an electrospray ionization-mass
spectrometry (ESI-MS) based analytical platform that provides high analytical throughput …