[HTML][HTML] Live-cell imaging in the deep learning era

JW Pylvänäinen, E Gómez-de-Mariscal… - Current Opinion in Cell …, 2023 - Elsevier
Live imaging is a powerful tool, enabling scientists to observe living organisms in real time.
In particular, when combined with fluorescence microscopy, live imaging allows the …

Fluorescence fluctuation‐based super‐resolution microscopy: Basic concepts for an easy start

A Alva, E Brito‐Alarcón, A Linares… - Journal of …, 2022 - Wiley Online Library
Due to the wave nature of light, optical microscopy has a lower‐bound lateral resolution limit
of approximately half of the wavelength of visible light, that is, within the range of 200 to 350 …

DBlink: Dynamic localization microscopy in super spatiotemporal resolution via deep learning

A Saguy, O Alalouf, N Opatovski, S Jang… - Nature …, 2023 - nature.com
Single-molecule localization microscopy (SMLM) has revolutionized biological imaging,
improving the spatial resolution of traditional microscopes by an order of magnitude …

[HTML][HTML] A mitochondrial NADPH-cholesterol axis regulates extracellular vesicle biogenesis to support hematopoietic stem cell fate

M Bonora, C Morganti, N van Gastel, K Ito, E Calura… - Cell Stem Cell, 2024 - cell.com
Mitochondrial fatty acid oxidation (FAO) is essential for hematopoietic stem cell (HSC) self-
renewal; however, the mechanism by which mitochondrial metabolism controls HSC fate …

Resolution enhancement with deblurring by pixel reassignment

B Zhao, J Mertz - Advanced photonics, 2023 - spiedigitallibrary.org
Improving the spatial resolution of a fluorescence microscope has been an ongoing
challenge in the imaging community. To address this challenge, a variety of approaches …

[HTML][HTML] Light-field flow cytometry for high-resolution, volumetric and multiparametric 3D single-cell analysis

X Hua, K Han, B Mandracchia, A Radmand… - Nature …, 2024 - nature.com
Imaging flow cytometry (IFC) combines flow cytometry and fluorescence microscopy to
enable high-throughput, multiparametric single-cell analysis with rich spatial details …

3D super-resolution live-cell imaging with radial symmetry and Fourier light-field microscopy

K Han, X Hua, V Vasani, GAR Kim, W Liu… - Biomedical Optics …, 2022 - opg.optica.org
Live-cell imaging reveals the phenotypes and mechanisms of cellular function and their
dysfunction that underscore cell physiology, development, and pathology. Here, we report a …

[HTML][HTML] Zero-shot learning enables instant denoising and super-resolution in optical fluorescence microscopy

C Qiao, Y Zeng, Q Meng, X Chen, H Chen… - Nature …, 2024 - nature.com
Computational super-resolution methods, including conventional analytical algorithms and
deep learning models, have substantially improved optical microscopy. Among them …

[HTML][HTML] Neural space–time model for dynamic multi-shot imaging

R Cao, NS Divekar, JK Nuñez, S Upadhyayula… - Nature …, 2024 - nature.com
Computational imaging reconstructions from multiple measurements that are captured
sequentially often suffer from motion artifacts if the scene is dynamic. We propose a neural …

[HTML][HTML] Harnessing artificial intelligence to reduce phototoxicity in live imaging

E Gómez-de-Mariscal, M Del Rosario… - Journal of Cell …, 2024 - journals.biologists.com
Fluorescence microscopy is essential for studying living cells, tissues and organisms.
However, the fluorescent light that switches on fluorescent molecules also harms the …