Labeling of nucleic acids is required for many studies aiming to elucidate their functions and
dynamics in vitro and in cells. Out of the numerous labeling concepts that have been …

Chemical modification of nucleic acids in living cells can be sterically hindered by tight
packing of bioorthogonal functional groups in chromatin. To address this limitation, we report …

Organic compounds that contain poly-triazole are very important intermediates in
pharmaceutical and chemical industry. Click chemistry is one of essential reactions that can …

Post-transcriptional regulation of gene expression occurs by multiple mechanisms, including
subcellular localization of mRNA and alteration of the poly (A) tail length. These …

Zebrafish (Danio rerio) is increasingly used to assess the pharmacological activity and
toxicity of compounds. The spatiotemporal distribution of seven fluorescent alkyne …

A modified nucleoside triphosphate bearing two modifications based on a 2′-deoxy-2′-
fluoro-arabinofuranose sugar and a uracil nucleobase equipped with a C5-ethynyl moiety (5 …

A requirement for biochemical labeling strategies is a pronounced biocompatibility of the
underlying reaction methodology. This protocol enables a systematic evaluation of the …

Fluorescent nucleoside triphosphates are powerful probes of DNA synthesis, but their
potential use in living animals has been previously underexplored. Here, we report the …

Genetically encoded protein or peptide tags and bioorthogonal chemistry are two growing
classes of tools in cell biological research. A protein or peptide tag could be genetically …

This era has witnessed the development and extensive application of modified nucleosides,
including fluorescent nucleosides that clinically served humankind. Most fluorescent …