Identifying circulating microRNAs as biomarkers of cardiovascular disease: a systematic review

R Navickas, D Gal, A Laucevičius… - Cardiovascular …, 2016 - academic.oup.com
The aim of the present study is to identify microRNAs (miRs) with high potential to be used
as biomarkers in plasma and/or serum to clinically diagnose, or provide accurate prognosis …

With reference to reference genes: a systematic review of endogenous controls in gene expression studies

JR Chapman, J Waldenström - PloS one, 2015 - journals.plos.org
The choice of reference genes that are stably expressed amongst treatment groups is a
crucial step in real-time quantitative PCR gene expression studies. Recent guidelines have …

Soybean photosynthesis and crop yield are improved by accelerating recovery from photoprotection

AP De Souza, SJ Burgess, L Doran, J Hansen… - Science, 2022 - science.org
Crop leaves in full sunlight dissipate damaging excess absorbed light energy as heat. This
protective dissipation continues after the leaf transitions to shade, reducing crop …

A TMPRSS2 inhibitor acts as a pan-SARS-CoV-2 prophylactic and therapeutic

T Shapira, IA Monreal, SP Dion, DW Buchholz… - Nature, 2022 - nature.com
The COVID-19 pandemic caused by the SARS-CoV-2 virus remains a global public health
crisis. Although widespread vaccination campaigns are underway, their efficacy is reduced …

Reduced physiological plasticity in a fish adapted to stable temperatures

R Morgan, AH Andreassen… - Proceedings of the …, 2022 - National Acad Sciences
Plasticity can allow organisms to maintain consistent performance across a wide range of
environmental conditions. However, it remains largely unknown how costly plasticity is and …

Diffusion and bulk flow of amino acids mediate calcium waves in plants

A Bellandi, D Papp, A Breakspear, J Joyce… - Science …, 2022 - science.org
In plants, a variety of stimuli trigger long-range calcium signals that travel rapidly along the
vasculature to distal tissues via poorly understood mechanisms. Here, we use quantitative …

RT-qPCR testing of SARS-CoV-2: a primer

SA Bustin, T Nolan - International journal of molecular sciences, 2020 - mdpi.com
Testing for the presence of coronavirus is an essential diagnostic tool for monitoring and
managing the current COVID-19 pandemic. The only reliable test in current use for testing …

[HTML][HTML] How good is a PCR efficiency estimate: Recommendations for precise and robust qPCR efficiency assessments

D Svec, A Tichopad, V Novosadova, MW Pfaffl… - Biomolecular detection …, 2015 - Elsevier
We have examined the imprecision in the estimation of PCR efficiency by means of standard
curves based on strategic experimental design with large number of technical replicates. In …

High‐efficiency gene targeting in hexaploid wheat using DNA replicons and CRISPR/Cas9

J Gil‐Humanes, Y Wang, Z Liang, Q Shan… - The Plant …, 2017 - Wiley Online Library
The ability to edit plant genomes through gene targeting (GT) requires efficient methods to
deliver both sequence‐specific nucleases (SSN s) and repair templates to plant cells. This is …

Simultaneous CRISPR/Cas9‐mediated editing of cassava eIF4E isoforms nCBP‐1 and nCBP‐2 reduces cassava brown streak disease symptom severity and …

MA Gomez, ZD Lin, T Moll, RD Chauhan… - Plant biotechnology …, 2019 - Wiley Online Library
Cassava brown streak disease (CBSD) is a major constraint on cassava yields in East and
Central Africa and threatens production in West Africa. CBSD is caused by two species of …