The study of proteins circulating in blood offers tremendous opportunities to diagnose,
stratify, or possibly prevent diseases. With recent technological advances and the urgent …

The data‐independent acquisition mass spectrometry (DIA‐MS) has rapidly evolved as a
powerful alternative for highly reproducible proteome profiling with a unique strength of …

The dia-PASEF technology uses ion mobility separation to reduce signal interferences and
increase sensitivity in proteomic experiments. Here we present a two-dimensional peak …

Single‐cell technologies are revolutionizing biology but are today mainly limited to imaging
and deep sequencing. However, proteins are the main drivers of cellular function and in …

Current mass spectrometry methods enable high-throughput proteomics of large sample
amounts, but proteomics of low sample amounts remains limited in depth and throughput. To …

Mass spectrometry (MS)-based proteomics aims to characterize comprehensive proteomes
in a fast and reproducible manner. Here we present the narrow-window data-independent …

Peptide identification in liquid chromatography-tandem mass spectrometry (LC-MS/MS)
experiments relies on computational algorithms for matching acquired MS/MS spectra …

Microbial communities are composed of cells of varying metabolic capacity, and regularly
include auxotrophs that lack essential metabolic pathways. Through analysis of auxotrophs …

Recent technological advances in mass spectrometry promise to add single-cell proteomics
to the biologist's toolbox. Here we discuss the current status and what is needed for this …

Mass spectrometry (MS)-based ubiquitinomics provides system-level understanding of
ubiquitin signaling. Here we present a scalable workflow for deep and precise in vivo …