Antibiotic resistance is a major global health challenge and, worryingly, several key Gram
negative pathogens can become resistant to most currently available antibiotics. Polymyxins …

The lipid A moiety of lipopolysaccharide forms the outer monolayer of the outer membrane of
most gram-negative bacteria. Escherichia coli lipid A is synthesized on the cytoplasmic …

His‐tag affinity purification is one of the most commonly used methods to purify recombinant
proteins expressed in E. coli. One drawback of using the His‐tag is the co‐purification of …

The ability of gram-negative bacteria to resist killing by antimicrobial agents and to avoid
detection by host immune systems often entails modification to the lipopolysaccharide (LPS) …

Nucleotide sugars are the universal sugar donors for the formation of polysaccharides,
glycoproteins, proteoglycans, glycolipids, and glycosylated secondary metabolites. At least …

Modification of lipid A with the 4-amino-4-deoxy-l-arabinose (l-Ara4N) moiety is required for
resistance to polymyxin and cationic antimicrobial peptides in Escherichia coli and …

This review examines the mechanisms propelling cofactor-independent, organic cofactor-
dependent and metal-dependent decarboxylase chemistry. Decarboxylation, the removal of …

Covering: up to 2012 The glycosylation of microbial natural products often dramatically
influences the biological and/or pharmacological activities of the parental metabolite. Over …

Lipooligosaccharides (LOSs) are antigenic glycolipids that are present in some species of
Mycobacterium including the Canetti strain of M. tuberculosis. The core LOS structures from …

GDP-mannose-3 ', 5 '-epimerase (GME) from Arabidopsis thaliana catalyzes the
epimerization of both the 3 'and 5 'positions of GDP-α-d-mannose to yield GDP-β-l …