[HTML][HTML] Strategies in the delivery of Cas9 ribonucleoprotein for CRISPR/Cas9 genome editing

S Zhang, J Shen, D Li, Y Cheng - Theranostics, 2021 - ncbi.nlm.nih.gov
CRISPR/Cas9 genome editing has gained rapidly increasing attentions in recent years,
however, the translation of this biotechnology into therapy has been hindered by efficient …

Gene drives gaining speed

E Bier - Nature Reviews Genetics, 2022 - nature.com
Gene drives are selfish genetic elements that are transmitted to progeny at super-Mendelian
(> 50%) frequencies. Recently developed CRISPR–Cas9-based gene-drive systems are …

Towards application of CRISPR-Cas12a in the design of modern viral DNA detection tools

J Dronina, U Samukaite-Bubniene… - Journal of …, 2022 - Springer
Early detection of viral pathogens by DNA-sensors in clinical samples, contaminated foods,
soil or water can dramatically improve clinical outcomes and reduce the socioeconomic …

DIPA-CRISPR is a simple and accessible method for insect gene editing

Y Shirai, MD Piulachs, X Belles, T Daimon - Cell Reports Methods, 2022 - cell.com
Current approaches for insect gene editing require microinjection of materials into early
embryos. This severely limits the application of gene editing to a great number of insect …

The application of DNA polymerases and Cas9 as representative of DNA-modifying enzymes group in DNA sensor design

J Dronina, US Bubniene, A Ramanavicius - Biosensors and Bioelectronics, 2021 - Elsevier
Rapid detection of nucleic acids (DNA or RNA) by inexpensive, selective, accurate, and
highly sensitive methods is very important for biosensors. DNA-sensors based on DNA …

CRISPR-Cas9-Based Genome Editing in the Silverleaf Whitefly (Bemisia tabaci)

CC Heu, FM McCullough, J Luan, JL Rasgon - The CRISPR journal, 2020 - liebertpub.com
Bemisia tabaci cryptic species Middle East-Asia Minor I (MEAM1) is a serious agricultural
polyphagous insect pest and vector of numerous plant viruses, causing major worldwide …

[HTML][HTML] Cas9-mediated gene editing in the black-legged tick, Ixodes scapularis, by embryo injection and ReMOT Control

A Sharma, MN Pham, JB Reyes, R Chana, WC Yim… - Iscience, 2022 - cell.com
Despite their capacity to acquire and pass on an array of debilitating pathogens, research on
ticks has lagged behind other arthropod vectors, such as mosquitoes, largely because of …

Functional genomic tools for emerging model species

E Gudmunds, CW Wheat, A Khila, A Husby - Trends in Ecology & Evolution, 2022 - cell.com
Most studies in the field of ecology and evolution aiming to connect genotype to phenotype
rarely validate identified loci using functional tools. Recent developments in RNA …

Prospects and pitfalls: next-generation tools to control mosquito-transmitted disease

EP Caragata, S Dong, Y Dong… - Annual review of …, 2020 - annualreviews.org
Mosquito-transmitted diseases, including malaria and dengue, are a major threat to human
health around the globe, affecting millions each year. A diverse array of next-generation …

[HTML][HTML] Targeted mutagenesis using CRISPR-Cas9 in the chelicerate herbivore Tetranychus urticae

W Dermauw, W Jonckheere, M Riga, I Livadaras… - Insect biochemistry and …, 2020 - Elsevier
The use of CRISPR-Cas9 has revolutionized functional genetic work in many organisms,
including more and more insect species. However, successful gene editing or genetic …