Essential factors, advanced strategies, challenges, and approaches involved for efficient expression of recombinant proteins in Escherichia coli
Producing recombinant proteins is a major accomplishment of biotechnology in the past
century. Heterologous hosts, either eukaryotic or prokaryotic, are used for the production of …
century. Heterologous hosts, either eukaryotic or prokaryotic, are used for the production of …
Amyloid fibril formation of Arctic amyloid-β 1–42 peptide is efficiently inhibited by the BRICHOS domain
X Zhong, R Kumar, Y Wang, H Biverstål… - ACS Chemical …, 2022 - ACS Publications
Amyloid-β peptide (Aβ) aggregation is one of the hallmarks of Alzheimer's disease (AD).
Mutations in Aβ are associated with early onset familial AD, and the Arctic mutant E22G …
Mutations in Aβ are associated with early onset familial AD, and the Arctic mutant E22G …
Production of recombinant HPV11/16 E6/E7-MBP-His6 fusion proteins and their potential to induce cytokine secretion by immune cells in peripheral blood
M Xu, M Zhong, S Feng, Y Xu, X Peng, K Zeng… - Virology Journal, 2024 - Springer
Human papillomavirus (HPV) infection poses a significant threat to public health worldwide.
Targeting the function of HPV E6 and E7 proteins and activating the host immune response …
Targeting the function of HPV E6 and E7 proteins and activating the host immune response …
[HTML][HTML] Advancing large-scale production of TEV protease through an innovative NT* tag-based fusion construct
PP Parida, D Saraswathi, SMV Mopidevi… - Current Research in …, 2023 - Elsevier
Abstract Tobacco etch virus Protease (TEVp), a cysteine protease, is renowned for its
remarkable specific proteolysis, making it an invaluable tool for removing fusion tags from …
remarkable specific proteolysis, making it an invaluable tool for removing fusion tags from …
[HTML][HTML] PhiC31 整合酶电穿孔鸡成纤维细胞诱发位点特异性基因盒交换
王辉， 许梦缘， 刘灵康， 郑喜邦， 李恭贺， 吴文德 - 畜牧兽医学报, 2023 - html.rhhz.net
旨在验证直接电穿孔重组蛋白PhiC31 能否在鸡DF-1 细胞中诱导PhiC31 介导的基因盒交换.
本研究通过原核表达和经亲和层析纯化获得SUMO-His-PhiC31 融合蛋白, 利用SUMO …
本研究通过原核表达和经亲和层析纯化获得SUMO-His-PhiC31 融合蛋白, 利用SUMO …
Functional and evolutionary constraints of wtf killer meiotic drivers
AN Srinivasa, S Campbell, S Venkatesan, NL Nuckolls… - bioRxiv, 2024 - biorxiv.org
Killer meiotic drivers are selfish DNA loci that sabotage the gametes that do not inherit them
from a driver+/driver-heterozygote. These drivers often employ toxic proteins that target …
from a driver+/driver-heterozygote. These drivers often employ toxic proteins that target …
Preparation and self-cleavage of fusion soluble farnesyl diphosphate synthase in E. coli
W Ni, Z Wang, A Zheng, Y Zhao - Preparative Biochemistry & …, 2023 - Taylor & Francis
Farnesyl diphosphate synthase (FPPS) is a crucial protein in terpenoid production.
However, its industrial application is limited owing to its low solubility in Escherichia coli. In …
However, its industrial application is limited owing to its low solubility in Escherichia coli. In …
Characterizing the Self Association of the Intrinsically Disordered Low-Complexity Domain of the RNA Binding Protein EWS
CN Johnson - 2023 - search.proquest.com
The RNA-binding protein EWS is a multifunctional protein with roles in the regulation of
transcription and RNA splicing. It is one of the FET (FUS, EWS and TAF15) family of RNA …
transcription and RNA splicing. It is one of the FET (FUS, EWS and TAF15) family of RNA …