Förster (or Fluorescence) Resonance Energy Transfer (FRET) is unique in generating
fluorescence signals sensitive to molecular conformation, association, and separation in the …

In fluorescence microscopy, the fluorescence emission can be characterised not only by
intensity and position, but also by lifetime, polarization and wavelength. Fluorescence …

This article describes an emerging method for quantitative measurement and spatial
imaging of microviscosity within individual domains of live cells. The method is based on …

The fluorescence intensity and lifetime of the 4, 4′-difluoro-4-bora-5-(p-oxoalkyl) phenyl-
3a, 4a-diaza-s-indacene (1) show a strong correlation with the viscosity of the medium due …

The cytoplasm is a crowded, visco-elastic environment whose physical properties change
according to physiological or developmental states. How the physical properties of the …

Fluorescence lifetime imaging microscopy (FLIM) is a technique to map the spatial
distribution of nanosecond excited state lifetimes within microscopic images. FLIM systems …

We report intracellular fluorescence lifetime imaging (FLIM) and fluorescence anisotropy
measurements of two meso-substituted fluorophores based on the boron− dipyrrin (BODIPY) …

Fluorescence lifetime imaging (FLIM) and fluorescence polarization imaging are
complementary techniques that can be used to extract information about macromolecules …

Fluorescence-anisotropy-based homo-FRET detection methods can be employed to study
clustering of identical proteins in cells. Here, the potential of fluorescence anisotropy …

The microscopic viscosity plays an essential role in cellular biophysics by controlling the
rates of diffusion and bimolecular reactions within the cell interior. While several approaches …