Industrial biotechnology of Pseudomonas putida: advances and prospects

A Weimer, M Kohlstedt, DC Volke, PI Nikel… - Applied Microbiology …, 2020 - Springer
Pseudomonas putida is a Gram-negative, rod-shaped bacterium that can be encountered in
diverse ecological habitats. This ubiquity is traced to its remarkably versatile metabolism …

[HTML][HTML] Pseudomonas putida as a functional chassis for industrial biocatalysis: from native biochemistry to trans-metabolism

PI Nikel, V de Lorenzo - Metabolic engineering, 2018 - Elsevier
The itinerary followed by Pseudomonas putida from being a soil-dweller and plant colonizer
bacterium to become a flexible and engineer-able platform for metabolic engineering stems …

Muconic acid production from glucose and xylose in Pseudomonas putida via evolution and metabolic engineering

C Ling, GL Peabody, D Salvachúa, YM Kim… - Nature …, 2022 - nature.com
Muconic acid is a bioprivileged molecule that can be converted into direct replacement
chemicals for incumbent petrochemicals and performance-advantaged bioproducts. In this …

Modular (de) construction of complex bacterial phenotypes by CRISPR/nCas9-assisted, multiplex cytidine base-editing

DC Volke, RA Martino, E Kozaeva, AM Smania… - Nature …, 2022 - nature.com
CRISPR/Cas technologies constitute a powerful tool for genome engineering, yet their use
in non-traditional bacteria depends on host factors or exogenous recombinases, which limits …

Bioremediation 3.0: engineering pollutant-removing bacteria in the times of systemic biology

P Dvořák, PI Nikel, J Damborský, V de Lorenzo - Biotechnology advances, 2017 - Elsevier
Elimination or mitigation of the toxic effects of chemical waste released to the environment
by industrial and urban activities relies largely on the catalytic activities of microorganisms …

Chasing bacterial chassis for metabolic engineering: a perspective review from classical to non‐traditional microorganisms

P Calero, PI Nikel - Microbial Biotechnology, 2019 - Wiley Online Library
The last few years have witnessed an unprecedented increase in the number of novel
bacterial species that hold potential to be used for metabolic engineering. Historically …

From lignin to nylon: cascaded chemical and biochemical conversion using metabolically engineered Pseudomonas putida

M Kohlstedt, S Starck, N Barton, J Stolzenberger… - Metabolic …, 2018 - Elsevier
Cis, cis-muconic acid (MA) is a chemical that is recognized for its industrial value and is
synthetically accessible from aromatic compounds. This feature provides the attractive …

Reconfiguration of metabolic fluxes in Pseudomonas putida as a response to sub-lethal oxidative stress

PI Nikel, T Fuhrer, M Chavarría… - The ISME …, 2021 - academic.oup.com
As a frequent inhabitant of sites polluted with toxic chemicals, the soil bacterium and plant-
root colonizer Pseudomonas putida can tolerate high levels of endogenous and exogenous …

The revisited genome of Pseudomonas putida KT2440 enlightens its value as a robust metabolic chassis

E Belda, RGA Van Heck… - Environmental …, 2016 - Wiley Online Library
By the time the complete genome sequence of the soil bacterium Pseudomonas putida
KT2440 was published in 2002 (Nelson et al.,) this bacterium was considered a potential …

Enhancing muconic acid production from glucose and lignin-derived aromatic compounds via increased protocatechuate decarboxylase activity

CW Johnson, D Salvachúa, P Khanna, H Smith… - Metabolic engineering …, 2016 - Elsevier
The conversion of biomass-derived sugars and aromatic molecules to cis, cis-muconic acid
(referred to hereafter as muconic acid or muconate) has been of recent interest owing to its …