A calcium-relay mechanism in vertebrate phototransduction

KW Koch, D Dell'Orco - ACS chemical neuroscience, 2013 - ACS Publications
Calcium-signaling in cells requires a fine-tuned system of calcium-transport proteins
involving ion channels, exchangers, and ion-pumps but also calcium-sensor proteins and …

The binding properties and physiological functions of recoverin

J Zang, SCF Neuhauss - Frontiers in molecular neuroscience, 2018 - frontiersin.org
Recoverin (Rcv) is a low molecular-weight, neuronal calcium sensor (NCS) primarily located
in photoreceptor outer segments of the vertebrate retina. Calcium ions (Ca2+)-bound Rcv …

Highly sensitive olfactory biosensors for the detection of volatile organic compounds by surface plasmon resonance imaging

C Hurot, S Brenet, A Buhot, E Barou, C Belloir… - Biosensors and …, 2019 - Elsevier
Nowadays, monitoring of volatile organic compounds (VOCs) is very important in various
domains. In this work, we aimed to develop sensitive olfactory biosensors using odorant …

Negative SPR signals during low molecular weight analyte recognition

H Bonnet, L Coche-Guérente, E Defrancq… - Analytical …, 2021 - ACS Publications
Surface plasmon resonance (SPR) is a powerful technique for studying biomolecular
interactions mainly due to its sensitivity and real-time and label free advantages. While SPR …

Biochemical and biophysical characterization of a plant calmodulin: Role of the N-and C-lobes in calcium binding, conformational change, and target interaction

A Astegno, V La Verde, V Marino, D Dell'Orco… - … et Biophysica Acta (BBA …, 2016 - Elsevier
In plants, transient elevation of intracellular Ca 2+ concentration in response to abiotic stress
is responsible for glutamate decarboxylase (GAD) activation via association with calmodulin …

Allosteric communication pathways routed by Ca2+/Mg2+ exchange in GCAP1 selectively switch target regulation modes

V Marino, D Dell'Orco - Scientific reports, 2016 - nature.com
GCAP1 is a neuronal calcium sensor protein that regulates the phototransduction cascade
in vertebrates by switching between activator and inhibitor of the target guanylate cyclase …

A novel p.(Glu111Val) missense mutation in GUCA1A associated with cone-rod dystrophy leads to impaired calcium sensing and perturbed second messenger …

V Marino, G Dal Cortivo, E Oppici… - Human Molecular …, 2018 - academic.oup.com
Abstract Guanylate Cyclase-Activating Protein 1 (GCAP1) regulates the enzymatic activity of
the photoreceptor guanylate cyclases (GC), leading to inhibition or activation of the cyclic …

Combining plasmonic and electrochemical biosensing methods

E Hemmerová, J Homola - Biosensors and Bioelectronics, 2024 - Elsevier
The idea of combining electrochemical (EC) and plasmonic biosensor methods was
introduced almost thirty years ago and the potential of electrochemical-plasmonic (EC-P) …

Do conformational changes contribute to the surface plasmon resonance signal?

D Dobrovodský, C Di Primo - Biosensors and Bioelectronics, 2023 - Elsevier
Surface plasmon resonance (SPR)-based biosensors are widely used instruments for
characterizing molecular interactions. In theory the SPR signal depends only on mass …

Two retinal dystrophy-associated missense mutations in GUCA1A with distinct molecular properties result in a similar aberrant regulation of the retinal guanylate …

V Marino, A Scholten, KW Koch… - Human Molecular …, 2015 - academic.oup.com
Two recently identified missense mutations (p. L84F and p. I107T) in GUCA1A, the gene
coding for guanylate cyclase (GC)-activating protein 1 (GCAP1), lead to a phenotype …