Coupled transcription-translation (CTT) is a hallmark of prokaryotic gene expression. CTT
occurs when ribosomes associate with and initiate translation of mRNAs whose transcription …

In recent years, transcriptional roadblocking has emerged as a crucial regulatory
mechanism in gene expression, whereby other DNA-bound obstacles can block the …

We used stochastic simulations and experimental data from E. coli, K. aerogenes,
Synechococcus PCC 7002 and Synechocystis PCC 6803 to provide evidence that …

Efficient and versatile DNA assembly frameworks have had an impact on promoting
synthetic biology to build complex biological systems. To accelerate system development …

Many prokaryotic RNAs are transcribed from loci outside of annotated protein coding genes.
Across bacterial species hundreds of short open reading frames antisense to annotated …

Closely spaced promoters in tandem formation are abundant in bacteria. We investigated
the evolutionary conservation, biological functions, and the RNA and single-cell protein …

Transcriptional interference (TI) has been shown to regulate gene expression at the DNA
level via different molecular mechanisms. The obstacles present on the DNA that a …

Antisense transcription is widespread in all kingdoms of life and has been shown to
influence gene expression through transcriptional interference (TI), a phenomenon in which …

Convergent promoters exert transcriptional interference (TI) by several mechanisms
including promoter occlusion, where elongating RNA polymerases (RNAP s) block access to …

Dynamic control is a distinguished strategy in modern metabolic engineering, in which
inducible convergent transcription is an attractive approach for conditional gene silencing …