The methylotrophic yeast Pichia pastoris (syn. Komagataella spp.) is one of the most
important production systems for heterologous proteins. After the first genome sequences …

The methylotrophic yeast Pichia pastoris (Komagataella spp.) is well established as a host
for recombinant protein production. Since a few years it has also been subject to metabolic …

Over the past 100 years, industrial biotechnology has grown into a multibillion dollar market,
which now has even begun to include large parts of chemical, material, and fuel production …

The promoter of the high-affinity glucose transporter Gth1 (P GTH1) is tightly repressed on
glucose and glycerol surplus, and strongly induced in glucose-limitation, thus enabling …

At specific growth rates above a particular critical value, Crabtree-positive microorganisms
exceed their respiratory capacity and enter diauxic growth metabolism. Excess substrate is …

Previously, we showed that the methylotrophic yeast Pichia pastoris (syn. Komagataella
phaffii) could produce and secrete the beta-propeller phytase FTEII in an active form under …

Pichia pastoris is recognized as a biotechnological workhorse for recombinant protein
expression. The metabolic performance of this microorganism depends on genetic makeup …

It is undisputed that stirred bioreactors are the most common type of bioreactors for
submerged bioprocesses. Stirred bioreactors for biopharmaceuticals, which represent the …

Summary The yeast Pichia pastoris (Komagataella sp.) is widely used as a production host
for recombinant proteins. Its ability to utilize methanol has largely dictated strain and process …

The methylotrophic yeast Komagataella phaffii (syn. Pichia pastoris) is a widely used host for
extracellularly producing heterologous proteins via an expression cassette integrated into …