Protein biopharmaceuticals such as monoclonal antibodies and therapeutic proteins are
currently in widespread use for the treatment of various life-threatening diseases including …

Antibody–drug conjugates (ADCs) are monoclonal antibodies with covalently bound
cytotoxic drugs. They are designed to target tumor antigens selectively and offer the hope of …

The reactive thiol in cysteine is used for coupling maleimide linkers in the generation of
antibody conjugates. To assess the impact of the conjugation site, we engineered cysteines …

Bioanalysis assays that reliably quantify biotherapeutics and biomarkers in biological
samples play pivotal roles in drug discovery and development. Liquid chromatography …

Disulfide bonds provide a bioactivatable connection with applications in imaging and
therapy. The circulation stability and intracellular release of disulfides are problematically …

THIOMAB antibody technology utilizes cysteine residues engineered onto an antibody to
allow for site-specific conjugation. The technology has enabled the exploration of different …

Antibody–drug conjugates (ADCs) typically consist of a cytotoxic drug covalently bound to
an antibody by a linker. These conjugates have the potential to substantially improve …

We present here a method for the rapid determination of the intact mass of noncovalently
associated antibody heavy chains (HC) and light chains (LC) which result from the …

Antibody drug conjugates (ADCs) are monoclonal antibodies designed to deliver a cytotoxic
drug selectively to antigen expressing cells. Several components of an ADC including the …

In this work, we utilize capillary electrophoresis–mass spectrometry (CE–MS) in an
integrated microfluidic platform to analyze an intact, lysine-linked antibody drug conjugate …