Embryo biopsies for genomic selection in tropical dairy cattle

CS Oliveira, LSA Camargo, MVGB Silva… - Animal …, 2023 - SciELO Brasil
Genomic selection has transformed the livestock industry, enabling early-life selection of
animals. Biopsy sampling of pre-implantation embryos has been described since 1968 …

In vitro-and in vivo-produced male dairy calves show molecular differences in the hepatic and muscular energy regulation

MB Rabaglino, JBM Secher, P Hyttel… - Biology of …, 2022 - academic.oup.com
In cattle, the in vitro production (IVP) of embryos is becoming more relevant than embryos
produced in vivo, ie after multiple ovulation and embryo transfer (MOET). However, the …

Recent advances of oocyte/embryo vitrification in mammals from rodents and large animals

M Kamoshita, H Sugita, A Kageyama… - Animal Science …, 2024 - Wiley Online Library
Vitrification is a valuable technology that enables semipermanent preservation and long‐
distance or international transportation of genetically modified and native animals. In …

Vitrification and in-straw warming do not affect pregnancy rates of biopsied bovine embryos

N González-Rodríguez, I Martínez-Rodero, J Scherzer… - Theriogenology, 2022 - Elsevier
In the cattle industry, in vivo or in vitro embryo production combined with genotyping and
cryopreservation technologies allows the selection and conservation of embryos carrying …

Blastocoel fluid aspiration improves vitrification outcomes and produces similar sexing results of in vitro-produced cattle embryos compared to microblade biopsy

I Martínez-Rodero, A Salas-Huetos, J Diaz-Muñoz… - Theriogenology, 2024 - Elsevier
The potential applications of in vitro-produced (IVP) cattle embryos are significantly
enhanced when combined with genotype selection and cryopreservation techniques. While …

Comparison of liquid nitrogen-free slow freezing protocols toward enabling a practical option for centralized cryobanking of ovarian tissue

E Asadi, A Najafi, JD Benson - Cryobiology, 2024 - Elsevier
Geographically distributed ovarian tissue cryobanks remain limited due to the high facility
and staff costs, and cold transportation to centers is associated with ischemia-induced tissue …

Cryopreservation increases accumulation of exogenous stearic acid in mouse embryos

AN Omelchenko, TN Igonina, EY Brusentsev… - Cryobiology, 2022 - Elsevier
Cryopreservation of preimplantation embryos is a widely used technique, but this procedure
might impact the subsequent embryo development. The effect of slow freezing and …

Use of Antifreeze Protein from Tenebrio molitor (TmAFP) in Vitrification of In Vitro–Produced Bovine Embryos: An Ultrastructural Study

RA Silva Júnior, R Desenzi, MMS Ramires… - Biopreservation and …, 2024 - liebertpub.com
The objective of this study was to evaluate the effects of different concentrations of antifreeze
protein (AFP) extracted from the larva of the beetle, Tenebrio molitor (Tm AFP), on …

Epigenomic and transcriptomic analyses reveal early activation of the HPG axis in in vitro-produced male dairy calves

MB Rabaglino, JBM Secher, MA Sirard, P Hyttel… - FASEB Journal, 2021 - orbit.dtu.dk
In cattle, several calves born after IVP (" in vitro" embryo production) present similar
birthweight to those generated after MOET (multiple ovulation and embryo transfer) …

Mitochondrial bioenergetic profiles of warmed bovine blastocysts are typically altered after cryopreservation by slow freezing and vitrification

J Kurzella, D Miskel, F Rings, E Tholen, D Tesfaye… - Theriogenology, 2024 - Elsevier
The widespread use of cryopreserved in vitro produced (IVP) bovine embryos is limited due
to their low post-warming viability compared to their ex vivo derived counterparts. Therefore …