Covalent Catalytic Strategies for Enzymes That Modify RNA Molecules on their Tripartite Building Blocks

CT Walsh - ACS Chemical Biology, 2022 - ACS Publications
The tripartite structures of the four 5′-nucleotide monophosphate (NMP) building blocks in
all RNAs enable enzyme-catalyzed chemical modifications to three types of sites: the …

Direct and noninvasive fluorescence analysis of an RNA-protein interaction based on a CRISPR/Cas12a-powered assay

X Wang, S Jing, W Wang, J Wang - Spectrochimica Acta Part A: Molecular …, 2023 - Elsevier
RNA-protein interactions (RPIs) play critical roles in gene transcription and protein
expression, but current analytical methods for RPIs are mainly performed in an invasive …

Assessing the in vitro Binding Affinity of Protein–RNA Interactions Using an RNA Pull-down Technique

A Chopra, F Balbous, KK Biggar - Bio-protocol, 2022 - bio-protocol.org
RNA is a vital component of the cell and is involved in a diverse range of cellular processes
through a variety of functions. However, many of these functions cannot be performed …

[HTML][HTML] Not making the cut: Techniques to prevent RNA cleavage in structural studies of RNase–RNA complexes

SP Jones, C Goossen, SD Lewis, AM Delaney… - Journal of Structural …, 2022 - Elsevier
RNases are varied in the RNA structures and sequences they target for cleavage and are an
important type of enzyme in cells. Despite the numerous examples of RNases known, and of …

Identification of Protein-RNA Interactions in Mouse Testis Tissue Using fRIP

AS Bailey, PJ Batista, HY Chang, MT Fuller - Bio-protocol, 2022 - bio-protocol.org
During development, cells must quickly switch from one cell state to the next to execute
precise and timely differentiation. One method to ensure fast transitions in cell states is by …

mRNPs biogenesis perturbation by the bacterial Rho factor: genomic analysis of the recrutment of the THO complex and its subunits

V Beauvais - 2023 - theses.hal.science
The transcription of messenger RNA (mRNA) is a complex process implicating a large
diversity of players at very specific steps. The production of an mRNA also requires its …