Nicotiana benthamiana: Its History and Future as a Model for Plant–Pathogen Interactions
MM Goodin, D Zaitlin, RA Naidu… - Molecular plant …, 2008 - Am Phytopath Society
Nicotiana benthamiana is the most widely used experimental host in plant virology, due
mainly to the large number of diverse plant viruses that can successfully infect it …
mainly to the large number of diverse plant viruses that can successfully infect it …
New dimensions for VIGS in plant functional genomics
M Senthil-Kumar, KS Mysore - Trends in plant science, 2011 - cell.com
Virus-induced gene silencing (VIGS) is an efficient tool for gene function studies. It has been
used to perform both forward and reverse genetics to identify plant genes involved in several …
used to perform both forward and reverse genetics to identify plant genes involved in several …
Tobacco rattle virus–based virus-induced gene silencing in Nicotiana benthamiana
M Senthil-Kumar, KS Mysore - Nature protocols, 2014 - nature.com
Tobacco rattle virus (TRV)-based virus-induced gene silencing (VIGS) is widely used in
various plant species to downregulate the expression of a target plant gene. TRV is a …
various plant species to downregulate the expression of a target plant gene. TRV is a …
RNA virus interference via CRISPR/Cas13a system in plants
Background CRISPR/Cas systems confer immunity against invading nucleic acids and
phages in bacteria and archaea. CRISPR/Cas13a (known previously as C2c2) is a class 2 …
phages in bacteria and archaea. CRISPR/Cas13a (known previously as C2c2) is a class 2 …
Validation of Reference Genes for Gene Expression Studies in Virus-Infected Nicotiana benthamiana Using Quantitative Real-Time PCR
Nicotiana benthamiana is the most widely-used experimental host in plant virology. The
recent release of the draft genome sequence for N. benthamiana consolidates its role as a …
recent release of the draft genome sequence for N. benthamiana consolidates its role as a …
CRISPR/Cas9-mediated genome editing and gene replacement in plants: transitioning from lab to field
SM Schaeffer, PA Nakata - Plant Science, 2015 - Elsevier
The CRISPR/Cas9 genome engineering system has ignited and swept through the scientific
community like wildfire. Owing largely to its efficiency, specificity, and flexibility, the …
community like wildfire. Owing largely to its efficiency, specificity, and flexibility, the …
VIGS–genomics goes functional
A Becker, M Lange - Trends in plant science, 2010 - cell.com
Genome and EST sequencing projects generate a wealth of sequence information for
agronomically and phylogenetically important plant species of which the majority is difficult …
agronomically and phylogenetically important plant species of which the majority is difficult …
The SGN VIGS tool: user-friendly software to design virus-induced gene silencing (VIGS) constructs for functional genomics
Virus-induced gene silencing (VIGS) is a fast and powerful method to study gene function in
plants (Burch-Smith et al., 2004). It is based on plant defense mechanisms against viral …
plants (Burch-Smith et al., 2004). It is based on plant defense mechanisms against viral …
CRISPR guides induce gene silencing in plants in the absence of Cas
Abstract Background RNA-targeting CRISPR-Cas can provide potential advantages over
DNA editing, such as avoiding pleiotropic effects of genome editing, providing precise …
DNA editing, such as avoiding pleiotropic effects of genome editing, providing precise …
Virus-induced gene silencing (VIGS) in Nicotiana benthamiana and tomato
AC Velásquez, S Chakravarthy… - Journal of visualized …, 2009 - pmc.ncbi.nlm.nih.gov
RNA interference (RNAi) is a highly specific gene-silencing phenomenon triggered by
dsRNA1. This silencing mechanism uses two major classes of RNA regulators: microRNAs …
dsRNA1. This silencing mechanism uses two major classes of RNA regulators: microRNAs …