Fluorescence imaging techniques play a pivotal role in our understanding of the nervous
system. The emergence of various super-resolution microscopy methods and specialized …

Correlative microscopy is a sophisticated approach that combines the capabilities of
typically separate, but powerful microscopy platforms: often including, but not limited, to …

We used electron tomography to examine microtubules (MTs) growing from pure tubulin in
vitro as well as two classes of MTs growing in cells from six species. The tips of all these …

The position and orientation of the mitotic spindle is precisely regulated to ensure the
accurate partition of the cytoplasm between daughter cells and the correct localization of the …

Many primary sensory cilia exhibit unique architectures that are critical for transduction of
specific sensory stimuli. Although basic ciliogenic mechanisms are well described, how …

Correlative light and electron microscopy (CLEM) is a powerful imaging approach that
allows the direct correlation of information obtained on a light and an electron microscope …

Interfaces between spindle microtubules and kinetochores were examined in diverse
species by electron tomography and image analysis. Overall structures were conserved in a …

High-pressure freezing (HPF) has been around since the mid-1980s as a cryopreparation
technique for biological electron microscopy. It has taken quite some time to “catch on” but …

In oocytes of many organisms, meiotic spindles form in the absence of centrosomes [1–5].
Such female meiotic spindles have a pointed appearance in metaphase with microtubules …

A method is described for the cryofixation of biological specimens for ultrastructural analysis
and immunocytochemical detection studies. The method employs plunge freezing of …