Reference genes in real-time PCR
B Kozera, M Rapacz - Journal of applied genetics, 2013 - Springer
This paper aims to discuss various aspects of the use of reference genes in qPCR technique
used in the thousands of present studies. Most frequently, these are housekeeping genes …
used in the thousands of present studies. Most frequently, these are housekeeping genes …
Quantitative real-time RT-PCR–a perspective
The real-time reverse transcription polymerase chain reaction (RT-PCR) uses fluorescent
reporter molecules to monitor the production of amplification products during each cycle of …
reporter molecules to monitor the production of amplification products during each cycle of …
Development and verification of the PAM50-based Prosigna breast cancer gene signature assay
B Wallden, J Storhoff, T Nielsen, N Dowidar… - BMC medical …, 2015 - Springer
Background The four intrinsic subtypes of breast cancer, defined by differential expression of
50 genes (PAM50), have been shown to be predictive of risk of recurrence and benefit of …
50 genes (PAM50), have been shown to be predictive of risk of recurrence and benefit of …
Normalization of circulating microRNA expression data obtained by quantitative real-time RT-PCR
F Marabita, P De Candia, A Torri… - Briefings in …, 2016 - academic.oup.com
The high-throughput analysis of microRNAs (miRNAs) circulating within the blood of healthy
and diseased individuals is an active area of biomarker research. Whereas quantitative real …
and diseased individuals is an active area of biomarker research. Whereas quantitative real …
Selection of internal control genes for quantitative real-time RT-PCR studies during tomato development process
M Expósito-Rodríguez, AA Borges, A Borges-Pérez… - BMC plant biology, 2008 - Springer
Background The elucidation of gene expression patterns leads to a better understanding of
biological processes. Real-time quantitative RT-PCR has become the standard method for …
biological processes. Real-time quantitative RT-PCR has become the standard method for …
A standard curve based method for relative real time PCR data processing
A Larionov, A Krause, W Miller - BMC bioinformatics, 2005 - Springer
Background Currently real time PCR is the most precise method by which to measure gene
expression. The method generates a large amount of raw numerical data and processing …
expression. The method generates a large amount of raw numerical data and processing …
Identification and validation of reference genes for quantitative RT-PCR normalization in wheat
AR Paolacci, OA Tanzarella, E Porceddu… - BMC molecular biology, 2009 - Springer
Background Usually the reference genes used in gene expression analysis have been
chosen for their known or suspected housekeeping roles, however the variation observed in …
chosen for their known or suspected housekeeping roles, however the variation observed in …
Normalization of microRNA expression levels in quantitative RT-PCR assays: identification of suitable reference RNA targets in normal and cancerous human solid …
HJ Peltier, GJ Latham - Rna, 2008 - rnajournal.cshlp.org
Proper normalization is a critical but often an underappreciated aspect of quantitative gene
expression analysis. This study describes the identification and characterization of …
expression analysis. This study describes the identification and characterization of …
Analytical validation of the PAM50-based Prosigna Breast Cancer Prognostic Gene Signature Assay and nCounter Analysis System using formalin-fixed paraffin …
T Nielsen, B Wallden, C Schaper, S Ferree, S Liu… - BMC cancer, 2014 - Springer
Abstract Background NanoString's Prosigna™ Breast Cancer Prognostic Gene Signature
Assay is based on the PAM50 gene expression signature. The test outputs a risk of …
Assay is based on the PAM50 gene expression signature. The test outputs a risk of …
Selection of reliable reference genes for gene expression studies in peach using real-time PCR
Z Tong, Z Gao, F Wang, J Zhou, Z Zhang - BMC molecular biology, 2009 - Springer
Background RT-qPCR is a preferred method for rapid and reliable quantification of gene
expression studies. Appropriate application of RT-qPCR in such studies requires the use of …
expression studies. Appropriate application of RT-qPCR in such studies requires the use of …