Organoids are three-dimensional (3D) miniature structures cultured in vitro produced from
either human pluripotent stem cells (hPSCs) or adult stem cells (AdSCs) derived from …

In vitro cell cultures are crucial research tools for modeling human development and
diseases. Although the conventional monolayer cell cultures have been widely used in the …

Recent advances in 3D culture technology allow embryonic and adult mammalian stem cells
to exhibit their remarkable self-organizing properties, and the resulting organoids reflect key …

The complexity of the human brain has made it difficult to study many brain disorders in
model organisms, highlighting the need for an in vitro model of human brain development …

Organoids are three-dimensional in-vitro-grown cell clusters with near-native microanatomy
that arise from self-organizing mammalian pluripotent or adult stem cells. Although …

Background Because of their differentiation potential, pluripotent stem cells can generate
virtually any cell type and, as such, can be used to model development and disease and …

During cerebellar development, the main portion of the cerebellar plate neuroepithelium
gives birth to Purkinje cells and interneurons, whereas the rhombic lip, the germinal zone at …

The development of the mammalian cerebellum is orchestrated by both cell-autonomous
programs and inductive environmental influences. Here, we describe the main processes of …

The ability to model human brain development in vitro represents an important step in our
study of developmental processes and neurological disorders. Protocols that utilize human …

Human brain organoid techniques have rapidly advanced to facilitate investigating human
brain development and diseases. These efforts have largely focused on generating …