Editing plant genomes with CRISPR/Cas9
K Belhaj, A Chaparro-Garcia, S Kamoun… - Current opinion in …, 2015 - Elsevier
Highlights•Cas9 is an RNA-guided DNA endonuclease innate to prokaryotic immune
systems.•CRISPR/Cas9 has recently emerged as a powerful genome editing …
systems.•CRISPR/Cas9 has recently emerged as a powerful genome editing …
Applying CRISPR/Cas for genome engineering in plants: the best is yet to come
H Puchta - Current opinion in plant biology, 2017 - Elsevier
Highlights•CRISPR/Cas mediated gene editing is becoming routine for plants.•Much more
can be achieved in future by further applications.•Gene editing will become more precise …
can be achieved in future by further applications.•Gene editing will become more precise …
Targeted genome modifications in soybean with CRISPR/Cas9
Background The ability to selectively alter genomic DNA sequences in vivo is a powerful tool
for basic and applied research. The CRISPR/Cas9 system precisely mutates DNA …
for basic and applied research. The CRISPR/Cas9 system precisely mutates DNA …
High‐efficiency gene targeting in hexaploid wheat using DNA replicons and CRISPR/Cas9
The ability to edit plant genomes through gene targeting (GT) requires efficient methods to
deliver both sequence‐specific nucleases (SSN s) and repair templates to plant cells. This is …
deliver both sequence‐specific nucleases (SSN s) and repair templates to plant cells. This is …
Both CRISPR/Cas‐based nucleases and nickases can be used efficiently for genome engineering in Arabidopsis thaliana
Engineered nucleases can be used to induce site‐specific double‐strand breaks (DSB s) in
plant genomes. Thus, homologous recombination (HR) can be enhanced and targeted …
plant genomes. Thus, homologous recombination (HR) can be enhanced and targeted …
CRISPR/Cas9‐mediated mutagenesis of lncRNA1459 alters tomato fruit ripening
R Li, D Fu, B Zhu, Y Luo, H Zhu - The Plant Journal, 2018 - Wiley Online Library
With the development of high‐throughput sequencing, many long non‐coding RNA s (lnc
RNA s) have been found to play important roles in diverse biological processes. However …
RNA s) have been found to play important roles in diverse biological processes. However …
Precision genome engineering and agriculture: opportunities and regulatory challenges
Plant agriculture is poised at a technological inflection point. Recent advances in genome
engineering make it possible to precisely alter DNA sequences in living cells, providing …
engineering make it possible to precisely alter DNA sequences in living cells, providing …
The CRISPR/Cas system can be used as nuclease for in planta gene targeting and as paired nickases for directed mutagenesis in Arabidopsis resulting in heritable …
The CRISPR/C as nuclease is becoming a major tool for targeted mutagenesis in
eukaryotes by inducing double‐strand breaks (DSB s) at pre‐selected genomic sites that are …
eukaryotes by inducing double‐strand breaks (DSB s) at pre‐selected genomic sites that are …
Plant breeding at the speed of light: the power of CRISPR/Cas to generate directed genetic diversity at multiple sites
Classical plant breeding was extremely successful in generating high yielding crop varieties.
Yet, in modern crops, the long domestication process has impoverished the genetic diversity …
Yet, in modern crops, the long domestication process has impoverished the genetic diversity …
Integration of Agrobacterium T-DNA into the Plant Genome
SB Gelvin - Annual review of genetics, 2017 - annualreviews.org
Agrobacterium strains transfer a single-strand form of T-DNA (T-strands) and Virulence (Vir)
effector proteins to plant cells. Following transfer, T-strands likely form complexes with Vir …
effector proteins to plant cells. Following transfer, T-strands likely form complexes with Vir …