Highlights•Major events in bacillus spore germination and outgrowth.•Molecular timeline
during spore germination and outgrowth.•Major molecular players in germination and recent …

Bacteria belonging to the orders Bacillales and Clostridiales form spores in response to
nutrient starvation. From a simplified morphological perspective, the spore can be …

Among all the–omics approaches, proteomics and metabolomics have received increased
attention over the last decade. Both approaches have reached a certain level of maturity …

Tandem mass spectrometry-based proteotyping allows characterizing microorganisms in
terms of taxonomy and is becoming an important tool for investigating microbial diversity …

Proteomic analysis by mass spectrometry of small (≤ 2 mg) solid tissue samples from
diverse formats requires high throughput and comprehensive proteome coverage. We …

2Duf, named after the presence of a transmembrane (TM) Duf421 domain and a small
Duf1657 domain in its sequence, is likely located in the inner membrane (IM) of spores in …

Aims This work aimed to characterize spore inner membrane (IM) properties and the
mechanism of spore killing by wet heat and H2O2 with spores overexpressing the 2Duf …

Bacillus subtilis spores can reactivate their metabolism through germination upon contact
with germinants and can develop into vegetative cells upon outgrowth. However, the …

Reliable and comprehensive multi-omics analysis is essential for researchers to understand
and explore complex biological systems more completely. Bacillus subtilis (B. subtilis) is a …

Resistant bacterial spores are a major concern in industrial decontamination processes. An
approach known as pressure-mediated germination-inactivation strategy aims to artificially …