In this Review article, we systematically summarize the design and applications of various
kinds of long-lived emissive probes for bioimaging and biosensing via time-resolved …

Fluorescence lifetime imaging (FLIM) is a key fluorescence microscopy technique to map the
environment and interaction of fluorescent probes. It can report on photophysical events that …

Photoluminescence lifetime imaging of upconverting nanoparticles is increasingly featured
in recent progress in optical thermometry. Despite remarkable advances in …

Lifetime imaging microscopy with sub-micron resolution provides essential understanding of
living systems by allowing both the visualisation of their structure, and the sensing of bio …

Image contrast is often limited by background autofluorescence in steady‐state bioimaging
microscopy. Upconversion bioimaging can overcome this by shifting the emission lifetime …

Saponins are a class of naturally occurring bioactive and biocompatible amphiphilic
glycosides produced by plants. Some saponins, such as α-hederin, exhibit unique cell …

The first transition‐metal complex‐based two‐photon absorbing luminescence lifetime
probes for cellular DNA are presented. This allows cell imaging of DNA free from …

Fluorescence imaging is the most widely used method for unveiling the molecular
composition of biological specimens. However, the weak optical emission of fluorescent …

Laser-scanning confocal fluorescence microscopy is an indispensable tool for biomedical
research by virtue of its high spatial resolution. Its temporal resolution is equally important …

Intensity-and amplitude-weighted average lifetimes, denoted as τ I and τ A hereafter, are
useful indicators for revealing Förster resonance energy transfer (FRET) or fluorescence …