The major facilitator superfamily (MFS) is the largest known superfamily of secondary active
transporters. MFS transporters are responsible for transporting a broad spectrum of …

Metabolic engineering aims to develop efficient cell factories by rewiring cellular
metabolism. As one of the most commonly used cell factories, Saccharomyces cerevisiae …

Cells can be programmed to monitor and react to their environment using genetic circuits.
Design automation software maps a desired circuit function to a DNA sequence, a process …

Whole-cell biocatalysts have proven a tractable path toward sustainable production of bulk
and fine chemicals. Yet the screening of libraries of cellular designs to identify best …

Genome-scale engineering is an indispensable tool to understand genome functions due to
our limited knowledge of cellular networks. Unfortunately, most existing methods for genome …

Adaptive laboratory evolution (ALE) has served as a historic microbial engineering method
that mimics natural selection to obtain desired microbes. The past decade has witnessed …

Biosensors are enabling major advances in the field of analytics that are both facilitating and
being facilitated by advances in synthetic biology. The ability of biosensors to rapidly and …

Allosteric transcription factors (aTFs) have proven widely applicable for biotechnology and
synthetic biology as ligand-specific biosensors enabling real-time monitoring, selection and …

Genetic sensors capable of converting key metabolite levels to fluorescence signals enable
the monitoring of intracellular compound concentrations in living cells, and emerge as an …

Highlights•Engineered cell experiences metabolic imbalance with reduced fitness.•
Overproduction phenotype requires dynamic redistribution of cellular resource.•Metabolite …