Alzheimer's disease (AD) is an age-related disease pathologically defined by the deposition
of amyloid plaques and neurofibrillary tangles in the brain parenchyma. Single-cell profiling …

Cell-based therapies are being developed for various neurodegenerative diseases that
affect the central nervous system (CNS). Concomitantly, the roles of individual cell types in …

Microglial reactivity is a pathological hallmark in many neurodegenerative diseases. During
stimulation, microglia undergo complex morphological changes, including loss of their …

Epigenome editing has rapidly evolved in recent years, with diverse applications that
include elucidating gene regulation mechanisms, annotating coding and noncoding …

CRISPR interference (CRISPRi) enables programmable, reversible, and titratable
repression of gene expression (knockdown) in mammalian cells. Initial CRISPRi-mediated …

Microglia are the tissue-resident macrophage population of the brain, specialized in
supporting the CNS environment and protecting it from endogenous and exogenous insults …

The CRISPR-Cas system is commonly known for its ability to cleave DNA in a
programmable manner, which has democratized gene editing and facilitated recent …

CRISPR-based technologies represent a major breakthrough in biomedical science as they
offer a powerful platform for unbiased screening and functional genomics in various fields …

Predicting the function of noncoding variation is a major challenge in modern genetics. In
this study, we used massively parallel reporter assays to screen 5706 variants identified …

Single-cell or single-nucleus transcriptomics is a powerful tool for identifying cell types and
cell states. However, hypotheses derived from these assays, including gene expression …