Amplification-free CRISPR/Cas detection technology: challenges, strategies, and perspectives

H Li, Y Xie, F Chen, H Bai, L Xiu, X Zhou… - Chemical Society …, 2023 - pubs.rsc.org
Rapid and accurate molecular diagnosis is a prerequisite for precision medicine, food
safety, and environmental monitoring. The clustered regularly interspaced short palindromic …

Point-of-care CRISPR/Cas nucleic acid detection: Recent advances, challenges and opportunities

JE van Dongen, JTW Berendsen… - Biosensors and …, 2020 - Elsevier
With the trend of moving molecular tests from clinical laboratories to on-site testing, there is a
need for nucleic acid based diagnostic tools combining the sensitivity, specificity and …

The CRISPR–Cas toolbox for analytical and diagnostic assay development

Y Tang, L Gao, W Feng, C Guo, Q Yang, F Li… - Chemical Society …, 2021 - pubs.rsc.org
Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and CRISPR-
associated (Cas) systems have revolutionized biological and biomedical sciences in many …

CRISPR‐Cas biochemistry and CRISPR‐based molecular diagnostics

Z Weng, Z You, J Yang, N Mohammad… - Angewandte Chemie …, 2023 - Wiley Online Library
Polymerase chain reaction (PCR)‐based nucleic acid testing has played a critical role in
disease diagnostics, pathogen surveillance, and many more. However, this method requires …

Clustered regularly interspaced short palindromic repeats/Cas9-mediated lateral flow nucleic acid assay

X Wang, E Xiong, T Tian, M Cheng, W Lin, H Wang… - ACS …, 2020 - ACS Publications
The lateral flow assay is one of the most convenient analytical techniques for analyzing the
immune response, but its applicability to precise genetic analyses is limited by the false …

CRISPR/cas systems redefine nucleic acid detection: principles and methods

M Wang, R Zhang, J Li - Biosensors and Bioelectronics, 2020 - Elsevier
Methods that enable rapid, sensitive and specific analyses of nucleic acid sequences have
positive effects on precise disease diagnostics and effective clinical treatments by providing …

CRISPR-based detection of SARS-CoV-2: A review from sample to result

R Nouri, Z Tang, M Dong, T Liu, A Kshirsagar… - Biosensors and …, 2021 - Elsevier
The current pandemic of the 2019 novel coronavirus (COVID-19) caused by SARS-CoV-2
(severe acute respiratory syndrome coronavirus-2) has raised significant public health …

Gold nanomaterials‐implemented CRISPR‐Cas systems for biosensing

R Fu, Y Xianyu - Small, 2023 - Wiley Online Library
Due to their superiority in the simple design and precise targeting, clustered regularly
interspaced short palindromic repeats (CRISPR)‐Cas systems have attracted significant …

Next-generation CRISPR-based diagnostic tools for human diseases

T Wang, Z Wang, L Bai, X Zhang, J Feng, C Qian… - TrAC Trends in …, 2023 - Elsevier
Accurate diagnosis of human diseases is crucial for effective prevention, clinical treatment,
and prognosis. The World Health Organization (WHO) has provided guidelines for molecular …

CRISPR/Cas-powered amplification-free detection of nucleic acids: current state of the art, challenges, and futuristic perspectives

Y Li, Y Liu, X Tang, J Qiao, J Kou, S Man, L Zhu… - ACS …, 2023 - ACS Publications
CRISPR/Cas system is becoming an increasingly influential technology that has been
repositioned in nucleic acid detection. A preamplification step is usually required to improve …