Pre-mRNA splicing is catalyzed by the spliceosome, a multimegadalton ribonucleoprotein
(RNP) complex comprised of five snRNPs and numerous proteins. Intricate RNA-RNA and …

The spliceosome, one of the most complex machineries of eukaryotic cells, removes intronic
sequences from primary transcripts to generate functional messenger and long noncoding …

During each cycle of pre-mRNA splicing, the pre-catalytic spliceosome (B complex) is
converted into the activated spliceosome (B act complex), which has a well-formed active …

Precursor mRNA (pre-mRNA) splicing proceeds by two consecutive transesterification
reactions via a lariat–intron intermediate. Here we present the 3.8 Å cryo-electron …

Superfamily 2 helicase proteins are ubiquitous in RNA biology and have an extraordinarily
broad set of functional roles. Central among these roles are the promotion of …

The spliceosome excises introns from pre-mRNAs in two sequential transesterifications—
branching and exon ligation—catalysed at a single catalytic metal site in U6 small nuclear …

During pre-mRNA splicing, a central step in the expression and regulation of eukaryotic
genes, the spliceosome selects splice sites for intron excision and exon ligation. In doing so …

Spliceosomal snRNPs are multicomponent particles that undergo a complex maturation
pathway. Human Sm-class snRNAs are generated as 3′-end extended precursors, which …

Noncoding introns are removed from nuclear precursor messenger RNA (pre-mRNA) in a
two-step phosphoryl transfer reaction by the spliceosome, a dynamic multimegadalton …

RNA helicases comprise a large family of enzymes that are thought to utilize the energy of
NTP binding and hydrolysis to remodel RNA or RNA-protein complexes, resulting in RNA …