Analytical ultracentrifugation: sedimentation velocity and sedimentation equilibrium

JL Cole, JW Lary, TP Moody, TM Laue - Methods in cell biology, 2008 - Elsevier
Analytical ultracentrifugation (AUC) is a versatile and powerful method for the quantitative
analysis of macromolecules in solution. AUC has broad applications for the study of …

Analytical ultracentrifugation of colloids

KL Planken, H Cölfen - Nanoscale, 2010 - pubs.rsc.org
In this contribution the use of Analytical Ultracentrifugation (AUC) for the modern analysis of
colloids is reviewed. Since AUC is a fractionation technique, distributions of the …

Structural basis of heterochromatin formation by human HP1

S Machida, Y Takizawa, M Ishimaru, Y Sugita, S Sekine… - Molecular cell, 2018 - cell.com
Heterochromatin plays important roles in transcriptional silencing and genome maintenance
by the formation of condensed chromatin structures, which determine the epigenetic status …

Histone H2B ubiquitylation disrupts local and higher-order chromatin compaction

B Fierz, C Chatterjee, RK McGinty… - Nature chemical …, 2011 - nature.com
Regulation of chromatin structure involves histone posttranslational modifications that can
modulate intrinsic properties of the chromatin fiber to change the chromatin state. We used …

Nucleosomal arrays self‐assemble into supramolecular globular structures lacking 30‐nm fibers

K Maeshima, R Rogge, S Tamura, Y Joti… - The EMBO …, 2016 - embopress.org
The existence of a 30‐nm fiber as a basic folding unit for DNA packaging has remained a
topic of active discussion. Here, we characterize the supramolecular structures formed by …

Novel mechanism of inhibition of rat kidney-type glutaminase by bis-2-(5-phenylacetamido-1, 2, 4-thiadiazol-2-yl) ethyl sulfide (BPTES)

MM Robinson, SJ Mcbryant, T Tsukamoto… - Biochemical …, 2007 - portlandpress.com
The release of GA (mitochondrial glutaminase) from neurons following acute ischaemia or
during chronic neurodegenerative diseases may contribute to the propagation of glutamate …

Structural bases of unphosphorylated STAT1 association and receptor binding

X Mao, Z Ren, GN Parker, H Sondermann… - Molecular cell, 2005 - cell.com
The crystal structure has been determined at 3.0 Å resolution for an unphosphorylated
STAT1 (1–683) complexed with a phosphopeptide derived from the α chain of interferon γ …

A two-dimensional spectrum analysis for sedimentation velocity experiments of mixtures with heterogeneity in molecular weight and shape

E Brookes, W Cao, B Demeler - European Biophysics Journal, 2010 - Springer
We report a model-independent analysis approach for fitting sedimentation velocity data
which permits simultaneous determination of shape and molecular weight distributions for …

UltraScan: a comprehensive data analysis software package for analytical ultracentrifugation experiments

B Demeler - Modern analytical ultracentrifugation: techniques …, 2005 - books.google.com
With the introduction of the Beckman XL-I/A in the early 1990s, digital data acquisition from
the analytical ultracentrifuge has made it possible to readily analyze sedimentation data with …

Structural and functional implications of the alternative complement pathway C3 convertase stabilized by a staphylococcal inhibitor

SHM Rooijakkers, J Wu, M Ruyken… - Nature …, 2009 - nature.com
Activation of the complement system generates potent chemoattractants and leads to the
opsonization of cells for immune clearance. Short-lived protease complexes cleave …