It has been 20 years since West Nile virus first emerged in the Americas, and since then,
little progress has been made to control outbreaks caused by this virus. After its first …

RNA viruses are unique in their evolutionary capacity, exhibiting high mutation rates and
frequent recombination. They rapidly adapt to environmental changes, such as shifts in …

How viruses evolve within hosts can dictate infection outcomes; however, reconstructing this
process is challenging. We evaluate our multiplexed amplicon approach, PrimalSeq, to …

West Nile virus (WNV) is an arthropod-borne, positive-sense RNA virus that poses an
increasing global threat due to warming climates and lack of effective therapeutics. Like …

In 2015, Zika virus (ZIKV; Flaviviridae; Flavivirus) emerged in the Americas, causing millions
of infections in dozens of countries. The rapid spread of the virus and the association with …

The emergence of mosquito-borne RNA viruses, such as West Nile virus (WNV), is facilitated
by genetically complex virus populations within hosts. Here, we determine whether WNV …

Due to their error-prone replication, RNA viruses typically exist as a diverse population of
closely related genomes, which is considered critical for their fitness and adaptive potential …

With next-generation sequencing technologies, it is now feasible to efficiently sequence
patient-derived virus populations at a depth of coverage sufficient to detect rare variants …

West Nile virus (WNV) continues to be a major cause of human arboviral neuroinvasive
disease. Susceptible non-human vertebrates are particularly diverse, ranging from …

The deterministic force of natural selection and stochastic influence of drift shape RNA virus
evolution. New deep-sequencing and microfluidics technologies allow us to quantify the …