Next-generation sequencing (NGS) technologies have greatly expanded the size of the
known transcriptome. Many newly discovered transcripts are classified as long noncoding …

The recent discovery of alternative open reading frames creates a need for suitable
analytical approaches to verify their translation and to characterize the corresponding gene …

Abstract1 Short open reading frame (sORF)–encoded polypeptides (SEPs) have recently
emerged as key regulators of major cellular processes. Computational methods for the …

Small proteins of up to∼ 50 amino acids are an abundant class of biomolecules across all
domains of life. Yet due to the challenges inherent in their size, they are often missed in …

The identification of proteins below approximately 70–100 amino acids in bottom-up
proteomics is still a challenging task due to the limited number of peptides generated by …

Advances in genomics and molecular biology have revealed an abundance of small open
reading frames (sORFs) across all types of transcripts. While these sORFs are often …

Proteins with up to 100 amino acids have been largely overlooked due to the challenges
associated with predicting and identifying them using traditional methods. Recent advances …

Small proteins play essential roles in bacterial physiology and virulence, however,
automated algorithms for genome annotation are often not yet able to accurately predict the …

Small open reading frames (sORFs) are often overlooked features in genomes. In the past,
they were labeled as noncoding or “transcriptional noise”. However, accumulating evidence …

Small proteins comprising less than 100 amino acids have been often ignored in bacterial
genome annotations. About 10 years ago, focused efforts started to investigate whole …