RNA processing plays a central role in accurately transmitting genetic information into
functional RNA and protein regulators. To fully appreciate the RNA life-cycle, tools to …

Alternative splicing is a widespread process contributing to structural transcript variation and
proteome diversity. In cancer, the splicing process is commonly disrupted, resulting in both …

Recent molecular studies have shown that, even when derived from a seemingly
homogenous population, individual cells can exhibit substantial differences in gene …

Considerable progress in sequencing technologies makes it now possible to study the
genomic and transcriptomic landscape of single cells. However, to better understand the …

Alternative splicing (AS) generates isoform diversity for cellular identity and homeostasis in
multicellular life. Although AS variation has been observed among single cells, little is …

Background RNA sequencing has opened new avenues for the study of transcriptome
composition. Significant evidence has accumulated showing that the human transcriptome …

Synthesis of mRNA in eukaryotes involves the coordinated action of many enzymatic
processes, including initiation, elongation, splicing, and cleavage. Kinetic competition …

Introns are removed from pre-mRNAs during transcription while the pre-mRNA is still
tethered to the gene locus via RNA polymerase. However, during alternative splicing, it is …

The field of single-cell biology has morphed from a philosophical digression at its inception,
to a playground for quantitative biologists, to a major area of biomedical research. The last …

Nearly all human multiexon genes are subject to alternative splicing in one or more cell
types. The splicing machinery therefore has to select between multiple splice sites in a …