Droplet microfluidics for microbiology: techniques, applications and challenges

TS Kaminski, O Scheler, P Garstecki - Lab on a Chip, 2016 - pubs.rsc.org
Droplet microfluidics has rapidly emerged as one of the key technologies opening up new
experimental possibilities in microbiology. The ability to generate, manipulate and monitor …

Microfluidic single‐cell omics analysis

X Xu, J Wang, L Wu, J Guo, Y Song, T Tian, W Wang… - Small, 2020 - Wiley Online Library
The commonly existing cellular heterogeneity plays a critical role in biological processes
such as embryonic development, cell differentiation, and disease progress. Single‐cell …

Mosaic patterned surfaces toward generating hardly‐volatile capsular droplet arrays for high‐precision droplet‐based storage and detection

L Jiao, Y Wu, Y Hu, Q Guo, H Wu, H Yu, L Deng, D Li… - Small, 2023 - Wiley Online Library
Precise detection involving droplets based on functional surfaces is promising for the
parallelization and miniaturization of platforms and is significant in epidemic investigation …

Fluorescence coupling strategies in fluorescence-activated droplet sorting (FADS) for ultrahigh-throughput screening of enzymes, metabolites, and antibodies

J Jiang, G Yang, F Ma - Biotechnology advances, 2023 - Elsevier
Fluorescence-activated droplet sorting (FADS) has emerged as a powerful tool for ultrahigh-
throughput screening of enzymes, metabolites, and antibodies. Fluorescence coupling …

Microfluidic Platforms for Real‐Time In Situ Monitoring of Biomarkers for Cellular Processes

C Lou, H Yang, Y Hou, H Huang, J Qiu… - Advanced …, 2024 - Wiley Online Library
Cellular processes are mechanisms carried out at the cellular level that are aimed at
guaranteeing the stability of the organism they comprise. The investigation of cellular …

Microfluidic technology for antibacterial resistance study and antibiotic susceptibility testing: review and perspective

N Qin, P Zhao, EA Ho, G Xin, CL Ren - ACS sensors, 2020 - ACS Publications
A review on microfluidic technology for antibacterial resistance study and antibiotic
susceptibility testing (AST) is presented here. Antibiotic resistance has become a global …

Homogenizing bacterial cell factories: analysis and engineering of phenotypic heterogeneity

D Binder, T Drepper, KE Jaeger, F Delvigne… - Metabolic …, 2017 - Elsevier
In natural habitats, microbes form multispecies communities that commonly face rapidly
changing and highly competitive environments. Thus, phenotypic heterogeneity has evolved …

Design and optimization of genetically encoded biosensors for high-throughput screening of chemicals

HG Lim, S Jang, S Jang, SW Seo, GY Jung - Current opinion in …, 2018 - Elsevier
Highlights•Genetically encoded biosensors are used in evolutionary metabolic
engineering.•These biosensors are constructed from natural or artificial genetic …

Recent advances in microfluidic single-cell analysis and its applications in drug development

Y Jiao, L Gao, Y Ji, W Liu - TrAC Trends in Analytical Chemistry, 2022 - Elsevier
Cellular heterogeneity is prevalent in all cell populations. Single-cell analysis (SCA) could
unveil the heterogeneity and thus gain unique insights into complex biological processes …

Development of Artificial Riboswitches for Monitoring of Naringenin In Vivo

S Jang, S Jang, Y Xiu, TJ Kang, SH Lee… - ACS synthetic …, 2017 - ACS Publications
Microbial strains are considered promising hosts for production of flavonoids because of
their rapid growth rate and suitability for large-scale manufacturing. However, productivity …