Super-resolution fluorescence imaging by photoactivation or photoswitching of single
fluorophores and position determination (single-molecule localization microscopy, SMLM) …

Correlative light and electron microscopy (CLEM) is a powerful imaging approach that
allows the direct correlation of information obtained on a light and an electron microscope …

Correlative electron and fluorescence microscopy has the potential to elucidate the
ultrastructural details of dynamic and rare cellular events, but has been limited by low …

Centrioles are necessary for flagella and cilia formation,, cytokinesis,, cell-cycle control and
centrosome organization/spindle assembly. They duplicate once per cell cycle, but the …

Fluorescence light microscopy (LM) has many advantages for the study of cell organization.
Specimen preparation is easy and relatively inexpensive, and the use of appropriate tags …

The dynamic instability of microtubules is a conserved and fundamental mechanism in
eukaryotes. Yet microtubules from different species diverge in their growth rates, lattice …

Here, we combine super-resolution fluorescence localization microscopy with scanning
electron microscopy to map the position of proteins of nuclear pore complexes in isolated …

The mitotic spindle ensures the faithful segregation of chromosomes. Here we combine the
first large-scale serial electron tomography of whole mitotic spindles in early C. elegans …

In metazoans, fertilization triggers the assembly of an extracellular coat that constitutes the
interface between the embryo and its environment. In nematodes, this coat is the eggshell …

The application of fluorescence and electron microscopy to the same specimen allows the
study of dynamic and rare cellular events at ultrastructural detail. Here, we present a …