Next-generation physiology approaches to study microbiome function at single cell level
The function of cells in their native habitat often cannot be reliably predicted from genomic
data or from physiology studies of isolates. Traditional experimental approaches to study the …
data or from physiology studies of isolates. Traditional experimental approaches to study the …
Bacterial growth efficiency in natural aquatic systems
PA Del Giorgio, JJ Cole - Annual Review of Ecology and …, 1998 - annualreviews.org
▪ Abstract Heterotrophic bacteria perform two major functions in the transformation of organic
matter: They produce new bacterial biomass (bacterial secondary production [BP]), and they …
matter: They produce new bacterial biomass (bacterial secondary production [BP]), and they …
Archaeal dominance in the mesopelagic zone of the Pacific Ocean
The ocean's interior is Earth's largest biome. Recently, cultivation-independent ribosomal
RNA gene surveys have indicated a potential importance for archaea in the subsurface …
RNA gene surveys have indicated a potential importance for archaea in the subsurface …
Natural Assemblages of Marine Proteobacteria and Members of the Cytophaga-Flavobacter Cluster Consuming Low- and High-Molecular-Weight Dissolved Organic …
MT Cottrell, DL Kirchman - Applied and environmental …, 2000 - Am Soc Microbiol
We used a method that combines microautoradiography with hybridization of fluorescent
rRNA-targeted oligonucleotide probes to whole cells (MICRO-FISH) to test the hypothesis …
rRNA-targeted oligonucleotide probes to whole cells (MICRO-FISH) to test the hypothesis …
Fluorescence in situ hybridization and catalyzed reporter deposition for the identification of marine bacteria
A Pernthaler, J Pernthaler, R Amann - Applied and environmental …, 2002 - Am Soc Microbiol
Fluorescence in situ hybridization (FISH) with horseradish peroxidase (HRP)-labeled
oligonucleotide probes and tyramide signal amplification, also known as catalyzed reporter …
oligonucleotide probes and tyramide signal amplification, also known as catalyzed reporter …
Microbial communities and their interactions in soil and rhizosphere ecosystems
AD Kent, EW Triplett - Annual Reviews in Microbiology, 2002 - annualreviews.org
▪ Abstract Since the first estimate of prokaryotic abundance in soil was published,
researchers have attempted to assess the abundance and distribution of species and relate …
researchers have attempted to assess the abundance and distribution of species and relate …
Contribution of Archaea to Total Prokaryotic Production in the Deep Atlantic Ocean
Fluorescence in situ hybridization (FISH) in combination with polynucleotide probes
revealed that the two major groups of planktonic Archaea (Crenarchaeota and …
revealed that the two major groups of planktonic Archaea (Crenarchaeota and …
Fluorescence in situ hybridization (FISH) with rRNA-targeted oligonucleotide probes
J Pernthaler, FO Glöckner, W Schönhuber… - Methods in …, 2001 - Elsevier
Publisher Summary The chapter discusses the fluorescence in situ hybridization (FISH) with
rRNA-targeted oligonucleotide probes, and describes probe designing and testing …
rRNA-targeted oligonucleotide probes, and describes probe designing and testing …
Significance of size and nucleic acid content heterogeneity as measured by flow cytometry in natural planktonic bacteria
Total bacterial abundances estimated with different epifluorescence microscopy methods
(4′, 6-diamidino-2-phenylindole [DAPI], SYBR Green, and Live/Dead) and with flow …
(4′, 6-diamidino-2-phenylindole [DAPI], SYBR Green, and Live/Dead) and with flow …
Compositional changes in free‐living bacterial communities along a salinity gradient in two temperate estuaries
TC Bouvier, PA del Giorgio - Limnology and oceanography, 2002 - Wiley Online Library
There is now clear evidence for major differences in heterotrophic bacterial composition
between freshwater and marine ecosystems. A fundamental question that remains …
between freshwater and marine ecosystems. A fundamental question that remains …