A new solution to the curved Ewald sphere problem for 3D image reconstruction in electron microscopy

JPJ Chen, KE Schmidt, JCH Spence, RA Kirian - Ultramicroscopy, 2021 - Elsevier
We develop an algorithm capable of imaging a three-dimensional object given a collection
of two-dimensional images of that object that are significantly influenced by the curvature of …

[HTML][HTML] Ghostbuster: A phase retrieval diffraction tomography algorithm for cryo-EM

J Yeo, BJ Daurer, D Kimanius, D Balakrishnan… - Ultramicroscopy, 2024 - Elsevier
Ewald sphere curvature correction, which extends beyond the projection approximation,
stretches the shallow depth of field in cryo-EM reconstructions of thick particles. Here we …

Method for virtual optical sectioning and tomography utilizing shallow depth of field

TE Gureyev, HM Quiney, LJ Allen - JOSA A, 2022 - opg.optica.org
A method is proposed for high-resolution, three-dimensional reconstruction of internal
structures of objects from planar transmission images. The described approach can be used …

Perspective: Emerging strategies for determining atomic-resolution structures of macromolecular complexes within cells

PN Petrov, H Müller, RM Glaeser - Journal of structural biology, 2022 - Elsevier
In principle, electron cryo-tomography (cryo-ET) of thin portions of cells provides high-
resolution images of the three-dimensional, spatial arrangement of all members of the …

A method for high-resolution three-dimensional reconstruction with Ewald sphere curvature correction from transmission electron images

TE Gureyev, DM Paganin, HG Brown… - Microscopy and …, 2022 - cambridge.org
A method for three-dimensional reconstruction of objects from defocused images collected
at multiple illumination directions in high-resolution transmission electron microscopy is …