Anything but conventional chromatography approaches in bioseparation

ACA Roque, AS Pina, AM Azevedo… - Biotechnology …, 2020 - Wiley Online Library
While packed bed chromatography, known as conventional chromatography, has been
serving the biopharmaceutical industry for decades as the bioseparation method of choice …

Nanomaterial-assisted microfluidics for multiplex assays

Y Wang, Y Gao, Y Yin, Y Pan, Y Wang, Y Song - Microchimica Acta, 2022 - Springer
Simultaneous detection of different biomarkers from a single specimen in a single test,
allowing more rapid, efficient, and low-cost analysis, is of great significance for accurate …

Sample-to-answer COVID-19 nucleic acid testing using a low-cost centrifugal microfluidic platform with bead-based signal enhancement and smartphone read-out

RRG Soares, AS Akhtar, IF Pinto, N Lapins, D Barrett… - Lab on a Chip, 2021 - pubs.rsc.org
With its origin estimated around December 2019 in Wuhan, China, the ongoing SARS-CoV-
2 pandemic is a major global health challenge. The demand for scalable, rapid and …

Transposing lateral flow immunoassays to capillary-driven microfluidics using self-coalescence modules and capillary-assembled receptor carriers

E Hemmig, Y Temiz, O Gökçe, RD Lovchik… - Analytical …, 2019 - ACS Publications
Point-of-care (POC) immunodiagnostic tests play a crucial role in enabling rapid and correct
diagnosis of diseases in prehospital care, emergency, and remote settings. In this work, we …

Sub-attomole detection of HIV-1 using padlock probes and rolling circle amplification combined with microfluidic affinity chromatography

RRG Soares, JC Varela, U Neogi, S Ciftci… - Biosensors and …, 2020 - Elsevier
Despite significant progress in diagnostics and disease management during the past
decades, human immunodeficiency virus (HIV) infections are still responsible for nearly 1 …

Silica bead-based microfluidic device with integrated photodiodes for the rapid capture and detection of rolling circle amplification products in the femtomolar range

RRG Soares, F Neumann, CRF Caneira… - Biosensors and …, 2019 - Elsevier
The rapid and sensitive detection of specific nucleic acid sequences at the point-of-care
(PoC) is becoming increasingly in demand for a variety of emergent biomedical applications …

Recent advances in mycotoxin determination for food monitoring via microchip

Y Man, G Liang, A Li, L Pan - Toxins, 2017 - mdpi.com
Mycotoxins are one of the main factors impacting food safety. Mycotoxin contamination has
threatened the health of humans and animals. Conventional methods for the detection of …

[HTML][HTML] A portable and low-cost centrifugal microfluidic platform for multiplexed colorimetric detection of protein biomarkers

AS Akhtar, RRG Soares, IF Pinto, A Russom - Analytica Chimica Acta, 2023 - Elsevier
Cytokines play a very important role in our immune system by acting as mediators to put up
a coordinated defense against foreign elements in our body. Elevated levels of cytokines in …

Recognition of nucleolin through interaction with RNA G-quadruplex

T Santos, A Miranda, MPC Campello, A Paulo… - Biochemical …, 2021 - Elsevier
The development of novel biomarkers for early-stage diagnosis of prostate cancer (PCa) has
attracted the attention of researchers in the last decade. Nucleolin (NCL) has emerged as a …

Multiplexed microfluidic fluorescence immunoassay with photodiode array signal acquisition for sub-minute and point-of-need detection of mycotoxins

RRG Soares, DR Santos, IF Pinto, AM Azevedo… - Lab on a Chip, 2018 - pubs.rsc.org
Portable, rapid, cost effective and simple analytical tools are in increasing demand to
facilitate the routine monitoring of target chemical/biological compounds at the point-of …