Generating mouse models for biomedical research: technological advances
CB Gurumurthy, KCK Lloyd - Disease models & …, 2019 - journals.biologists.com
Over the past decade, new methods and procedures have been developed to generate
genetically engineered mouse models of human disease. This At a Glance article highlights …
genetically engineered mouse models of human disease. This At a Glance article highlights …
Optimized CRISPR guide RNA design for two high-fidelity Cas9 variants by deep learning
Highly specific Cas9 nucleases derived from SpCas9 are valuable tools for genome editing,
but their wide applications are hampered by a lack of knowledge governing guide RNA …
but their wide applications are hampered by a lack of knowledge governing guide RNA …
Atlantic salmon (Salmo salar L.) genetics in the 21st century: taking leaps forward in aquaculture and biological understanding
RD Houston, DJ Macqueen - Animal Genetics, 2019 - Wiley Online Library
Atlantic salmon (Salmo salar L.) is among the most iconic and economically important fish
species and was the first member of Salmonidae to have a high‐quality reference genome …
species and was the first member of Salmonidae to have a high‐quality reference genome …
Whole genome sequencing reveals rare off‐target mutations and considerable inherent genetic or/and somaclonal variations in CRISPR/Cas9‐edited cotton plants
J Li, H Manghwar, L Sun, P Wang… - Plant biotechnology …, 2019 - Wiley Online Library
The CRISPR/Cas9 system has been extensively applied for crop improvement. However,
our understanding of Cas9 specificity is very limited in Cas9‐edited plants. To identify on …
our understanding of Cas9 specificity is very limited in Cas9‐edited plants. To identify on …
Cytosine base editor 4 but not adenine base editor generates off-target mutations in mouse embryos
Deaminase base editing has emerged as a tool to install or correct point mutations in the
genomes of living cells in a wide range of organisms. However, the genome-wide off-target …
genomes of living cells in a wide range of organisms. However, the genome-wide off-target …
[HTML][HTML] Anticipating and identifying collateral damage in genome editing
Genome editing has powerful applications in research, healthcare, and agriculture.
However, the range of possible molecular events resulting from genome editing has been …
However, the range of possible molecular events resulting from genome editing has been …
Variability in genome editing outcomes: challenges for research reproducibility and clinical safety
Genome editing tools have already revolutionized biomedical research and are also
expected to have an important impact in the clinic. However, their extensive use in research …
expected to have an important impact in the clinic. However, their extensive use in research …
Technologies and computational analysis strategies for CRISPR applications
The CRISPR-Cas system offers a programmable platform for eukaryotic genome and
epigenome editing. The ability to perform targeted genetic and epigenetic perturbations …
epigenome editing. The ability to perform targeted genetic and epigenetic perturbations …
Efficient and Specific Generation of MSTN-Edited Hu Sheep Using C-CRISPR
R Guo, H Wang, C Meng, H Gui, Y Li, F Chen, C Zhang… - Genes, 2023 - mdpi.com
Hu sheep, an indigenous breed in China known for its high fecundity, are being studied to
improve their growth and carcass traits. MSTN is a negative regulator of muscle …
improve their growth and carcass traits. MSTN is a negative regulator of muscle …
Illuminating the genome-wide activity of genome editors for safe and effective therapeutics
Genome editing holds remarkable promise to transform human medicine as new therapies
that can directly address the genetic causes of disease. However, concerns remain about …
that can directly address the genetic causes of disease. However, concerns remain about …