[HTML][HTML] Tag-mediated single-step purification and immobilization of recombinant proteins toward protein-engineered advanced materials

AI Freitas, L Domingues, TQ Aguiar - Journal of Advanced Research, 2022 - Elsevier
Background The potential applications of protein-engineered functional materials are so
wide and exciting that the interest in these eco-friendly advanced materials will further …

Biomimetic and bioinspired silicifications: Recent advances for biomaterial design and applications

MAA Abdelhamid, SP Pack - Acta Biomaterialia, 2021 - Elsevier
The rational design and controllable synthesis of functional silica-based materials have
gained increased interest in a variety of biomedical and biotechnological applications due to …

Opportunities and challenges of the tag-assisted protein purification techniques: Applications in the pharmaceutical industry

MM Gomari, N Saraygord-Afshari, M Farsimadan… - Biotechnology …, 2020 - Elsevier
Tag-assisted protein purification is a method of choice for both academic researches and
large-scale industrial demands. Application of the purification tags in the protein production …

Nanoparticle-mediated assembly of peptoid nanosheets functionalized with solid-binding proteins: designing heterostructures for hierarchy

J Ma, B Cai, S Zhang, T Jian, JJ De Yoreo, CL Chen… - Nano …, 2021 - ACS Publications
The fabrication of ordered architectures that intimately integrate polymer, protein, and
inorganic components remains difficult. Two promising building blocks to tackle this …

Recombinant protein purification and immobilization strategies based on peptides with dual affinity to iron oxide and silica

TQ Aguiar, L Domingues - Biotechnology Journal, 2023 - Wiley Online Library
Iron oxide and silica‐based materials have emerged as attractive protein purification and
immobilization matrices. His6 has been reported as an effective affinity tag for both iron …

Superior binding of proteins on a silica surface: Physical insight into the synergetic contribution of polyhistidine and a silica-binding peptide

C Liu, DL Steer, H Song, L He - The Journal of Physical Chemistry …, 2022 - ACS Publications
Controllable protein attachment onto solid interfaces is essential for the functionality of
proteins with broad applications. Silica-binding peptides (SBPs) have emerged as an …

Oriented multivalent silaffin-affinity immobilization of recombinant lipase on diatom surface: Reliable loading and high performance of biocatalyst

MAA Abdelhamid, RG Son, KS Park, SP Pack - Colloids and Surfaces B …, 2022 - Elsevier
Microbial lipases are widely used biocatalysts; however, their functional surface
immobilization should be designed for successful industrial applications. One of the unmet …

Ultrahigh adhesion force between silica-binding peptide SB7 and glass substrate studied by single-molecule force spectroscopy and molecular dynamic simulation

X Zhang, J Chen, E Li, C Hu, SZ Luo, C He - Frontiers in chemistry, 2020 - frontiersin.org
Many proteins and peptides have been identified to effectively and specifically bind on
certain surfaces such as silica, polystyrene and titanium dioxide. It is of great interest, in …

Predictive theoretical framework for dynamic control of bioinspired hybrid nanoparticle self-assembly

X Qi, Y Zhao, K Lachowski, J Boese, Y Cai, O Dollar… - ACS …, 2022 - ACS Publications
At-will tailoring of the formation and reconfiguration of hierarchical structures is a key goal of
modern nanomaterial design. Bioinspired systems comprising biomacromolecules and …

Sequence–structure–binding relationships reveal adhesion behavior of the Car9 solid-binding peptide: an integrated experimental and simulation study

B Hellner, S Alamdari, H Pyles, S Zhang… - Journal of the …, 2020 - ACS Publications
Solid-binding peptides (SBPs) recognizing inorganic and synthetic interfaces have enabled
a broad range of materials science applications and hold promise as adhesive or …