Colorectal cancer (CRC) is one of the commonest cancers globally. A unique aspect of CRC
is its intimate association with the gut microbiota, which forms an essential part of the tumour …

Despite the surge of microbial genome data, experimental testing is important to confirm
inferences about the cell biology, ecological roles and evolution of microorganisms. As the …

Pure bacterial cultures remain essential for detailed experimental and mechanistic studies in
microbiome research, and traditional methods to isolate individual bacteria from complex …

TH 2 cells and innate lymphoid cells 2 (ILC2) can stimulate tumor growth by secreting pro-
tumorigenic cytokines such as interleukin-4 (IL-4), IL-5, and IL-13. However, the …

Microbial culturing and meta-omic profiling technologies have significantly advanced our
understanding of the taxonomic and functional variation of the human microbiome and its …

Microbiomes occupy a range of niches and, in addition to having diverse compositions, they
have varied functional roles that have an impact on agriculture, environmental sciences, and …

Abstract Background The phylum Chloroflexi is highly abundant in a wide variety of
wastewater treatment bioreactors. It has been suggested that they play relevant roles in …

Owing to its ability to isolate single cells and perform high-throughput sorting, droplet sorting
has been widely applied in several research fields. Compared with flow cytometry, droplet …

Stony corals build the framework of coral reefs, ecosystems of immense ecological and
economic importance. The existence of these ecosystems is threatened by climate change …

Many microorganisms are auxotrophic—unable to synthesize the compounds they require
for growth. With this work, we quantify the prevalence of amino acid auxotrophies across a …