This review surveys the processes leading to loss of protein function and the types of
molecular interaction that help stabilize proteins. It considers the effects of organic solvents …

Chemical modification of enzymes and immobilization used to be considered as separate
ways to improve enzyme properties. This review shows how the coupled use of both tools …

The use of enzymes in waste treatment processes has been proposed by a number of
investigators. However, most of this work has focused on demonstrating the disappearance …

The maintenance or stabilization of protein or enzyme function is of vital importance in
Biotechnology. Investigations of thermophilic organisms, studies of denaturation and the use …

Horseradish peroxidase finds a variety of uses in analysis, immunology, organic synthesis,
and biosensors. Although moderately stable, its applicability to biosensors and other fields …

Thermal stability of horseradish peroxidase (HRP) has been enhanced by acetylation with
acetic acid n-hydroxysuccinimide ester (AA-NHS) under mild conditions. The half-life at 65° …

Enzynlcs Conn a class of natural catalysts\vith inleresting features. They can be selective for
the suhstnlle and specific"-'ithregard to thecata) ysed reaclion. Inaddition they 1l1ay be …

An alkali stable cellulase was obtained by chemical modification with 0.5 M maleic
anhydride at 2–4° C and pH 8.0 for 25min. The cellulase so modified showed a marginally …

Lyophilization, or freeze-drying, is a method for the preservation of labile materials in a
dehydrated form. It can be particularly suitable for high-value biomolecules such as proteins …

Alanine aminotransferase (ALT) is used in clinical diagnostics, amino acid synthesis and in
biosensors. Here we describe the stabilization of soluble porcine ALT by chemical …