Integrative methods and practical challenges for single-cell multi-omics
Fast-developing single-cell multimodal omics (scMulti-omics) technologies enable the
measurement of multiple modalities, such as DNA methylation, chromatin accessibility, RNA …
measurement of multiple modalities, such as DNA methylation, chromatin accessibility, RNA …
Development of therapeutic antibodies for the treatment of diseases
Z Wang, G Wang, H Lu, H Li, M Tang, A Tong - Molecular biomedicine, 2022 - Springer
Since the first monoclonal antibody drug, muromonab-CD3, was approved for marketing in
1986, 165 antibody drugs have been approved or are under regulatory review worldwide …
1986, 165 antibody drugs have been approved or are under regulatory review worldwide …
Human T-bet governs the generation of a distinct subset of CD11chighCD21low B cells
High-level expression of the transcription factor T-bet characterizes a phenotypically distinct
murine B cell population known as “age-associated B cells”(ABCs). T-bet–deficient mice …
murine B cell population known as “age-associated B cells”(ABCs). T-bet–deficient mice …
Refining colorectal cancer classification and clinical stratification through a single-cell atlas
Background Colorectal cancer (CRC) consensus molecular subtypes (CMS) have different
immunological, stromal cell, and clinicopathological characteristics. Single-cell …
immunological, stromal cell, and clinicopathological characteristics. Single-cell …
Bone marrow Adipoq-lineage progenitors are a major cellular source of M-CSF that dominates bone marrow macrophage development, osteoclastogenesis, and bone …
M-CSF is a critical growth factor for myeloid lineage cells, including monocytes,
macrophages, and osteoclasts. Tissue-resident macrophages in most organs rely on local M …
macrophages, and osteoclasts. Tissue-resident macrophages in most organs rely on local M …
Progress and challenges in mass spectrometry-based analysis of antibody repertoires
Humoral immunity is divided into the cellular B cell and protein-level antibody responses.
High-throughput sequencing has advanced our understanding of both these fundamental …
High-throughput sequencing has advanced our understanding of both these fundamental …
scRNA-seq revealed the special TCR β & α V (D) J allelic inclusion rearrangement and the high proportion dual (or more) TCR-expressing cells
L Zhu, Q Peng, J Li, Y Wu, J Wang, D Zhou, L Ma… - Cell Death & …, 2023 - nature.com
Allelic exclusion, one lymphocyte expresses one antigen receptor, is a fundamental
mechanism of immunological self-tolerance and highly specific immune responses to …
mechanism of immunological self-tolerance and highly specific immune responses to …
[HTML][HTML] A novel phenotype of B cells associated with enhanced phagocytic capability and chemotactic function after ischemic stroke
R Wang, H Li, C Ling, X Zhang, J Lu… - Neural Regeneration …, 2023 - journals.lww.com
Accumulating evidence has demonstrated the involvement of B cells in neuroinflammation
and neuroregeneration. However, the role of B cells in ischemic stroke remains unclear. In …
and neuroregeneration. However, the role of B cells in ischemic stroke remains unclear. In …
Global characterization of B cell receptor repertoire in COVID-19 patients by single-cell V (D) J sequencing
X Jin, W Zhou, M Luo, P Wang, Z Xu, K Ma… - Briefings in …, 2021 - academic.oup.com
The world is facing a pandemic of Corona Virus Disease 2019 (COVID-19) caused by
severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Adaptive immune …
severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Adaptive immune …
[HTML][HTML] Hybridoma technology: is it still useful?
JZ Moraes, B Hamaguchi, C Braggion… - Current research in …, 2021 - Elsevier
The isolation of single monoclonal antibodies (mAbs) against a given antigen was only
possible with the introduction of the hybridoma technology, which is based on the fusion of …
possible with the introduction of the hybridoma technology, which is based on the fusion of …