Discovery of a Ni2+-dependent guanidine hydrolase in bacteria

D Funck, M Sinn, JR Fleming, M Stanoppi, J Dietrich… - Nature, 2022 - nature.com
Nitrogen availability is a growth-limiting factor in many habitats, and the global nitrogen
cycle involves prokaryotes and eukaryotes competing for this precious resource. Only some …

Solving the conundrum: widespread proteins annotated for urea metabolism in bacteria are carboxyguanidine deiminases mediating nitrogen assimilation from …

NO Schneider, LJ Tassoulas, D Zeng, AJ Laseke… - Biochemistry, 2020 - ACS Publications
Free guanidine is increasingly recognized as a relevant molecule in biological systems.
Recently, it was reported that urea carboxylase acts preferentially on guanidine, and …

Bacterial catabolism of s-triazine herbicides: biochemistry, evolution and application

L Esquirol, TS Peat, E Sugrue, S Balotra… - Advances in microbial …, 2020 - Elsevier
The synthetic s-triazines are abundant, nitrogen-rich, heteroaromatic compounds used in a
multitude of applications including, herbicides, plastics and polymers, and explosives. Their …

Detecting low concentrations of nitrogen-based adulterants in whey protein powder using benchtop and handheld NIR spectrometers and the feasibility of scanning …

JL Zinia Zaukuu, B Aouadi, M Lukács, Z Bodor, F Vitális… - Molecules, 2020 - mdpi.com
Nitrogen-rich adulterants in protein powders present sensitivity challenges to conventional
combustion methods of protein determination which can be overcome by near Infrared …

Urea is a drop-in nitrogen source alternative to ammonium sulphate in Yarrowia lipolytica

O Konzock, S Zaghen, J Fu, EJ Kerkhoven - Iscience, 2022 - cell.com
Media components, including the nitrogen source, are significant cost factors in cultivation
processes. The nitrogen source also influences cell behavior and production performance …

The establishment of new protein expression system using N starvation inducible promoters in Chlorella

JH Shin, J Choi, J Jeon, M Kumar, J Lee, WJ Jeong… - Scientific Reports, 2020 - nature.com
Chlorella is a unicellular green microalga that has been used in fields such as bioenergy
production and food supplementation. In this study, two promoters of N (nitrogen) deficiency …

Efficient expression and purification of rat CRP in Pichia pastoris

B Cheng, YL Tang, YF Gou, JY Li, TH Xu… - Frontiers in …, 2024 - frontiersin.org
C-reactive protein (CRP) plays a crucial role in the diagnosis and monitoring of the non-
specific acute phase response in humans. In contrast, rat CRP (rCRP) is an atypical acute …

Diversity and ecological function of urease-producing bacteria in the cultivation environment of Gracilariopsis lemaneiformis

P Pei, M Aslam, H Wang, P Ye, T Li, H Liang, Q Lin… - Microbial Ecology, 2024 - Springer
Urease-producing bacteria (UPB) provide inorganic nitrogen for primary producers by
hydrolyzing urea, and play an important role in marine nitrogen cycle. However, there is still …

Role of a Two-Component Signal Transduction System RspA1/A2 in Regulating the Biosynthesis of Salinomycin in Streptomyces albus

K Zhang, A Mohsin, Y Dai, MF Ali, Z Chen… - Applied Biochemistry …, 2021 - Springer
The two-component system “AfsQ1/Q2” plays a crucial role to activate the production of
antibiotics ACT, RED, and CDA through directly binding the promoters of pathway-specific …

A novel decarboxylating amidohydrolase involved in avoiding metabolic dead ends during cyanuric acid catabolism in Pseudomonas sp. strain ADP

L Esquirol, TS Peat, M Wilding, CJ Hartley, J Newman… - Plos one, 2018 - journals.plos.org
Cyanuric acid is a common environmental contaminant and a metabolic intermediate in the
catabolism of s-triazine compounds, including atrazine and other herbicides. Cyanuric acid …