Serial femtosecond crystallography

TRM Barends, B Stauch, V Cherezov… - Nature reviews Methods …, 2022 - nature.com
With the advent of X-ray free-electron lasers (XFELs), new, high-throughput serial
crystallography techniques for macromolecular structure determination have emerged …

Serial millisecond crystallography for routine room-temperature structure determination at synchrotrons

T Weinert, N Olieric, R Cheng, S Brünle… - Nature …, 2017 - nature.com
Historically, room-temperature structure determination was succeeded by cryo-
crystallography to mitigate radiation damage. Here, we demonstrate that serial millisecond …

MyD88 TIR domain higher-order assembly interactions revealed by microcrystal electron diffraction and serial femtosecond crystallography

MTB Clabbers, S Holmes, TW Muusse… - Nature …, 2021 - nature.com
MyD88 and MAL are Toll-like receptor (TLR) adaptors that signal to induce pro-inflammatory
cytokine production. We previously observed that the TIR domain of MAL (MALTIR) forms …

[HTML][HTML] XFELs for structure and dynamics in biology

JCH Spence - IUCrJ, 2017 - scripts.iucr.org
The development and application of the free-electron X-ray laser (XFEL) to structure and
dynamics in biology since its inception in 2009 are reviewed. The research opportunities …

[PDF][PDF] Serial millisecond crystallography of membrane and soluble protein microcrystals using synchrotron radiation

JM Martin-Garcia, CE Conrad, G Nelson, N Stander… - IUCrJ, 2017 - journals.iucr.org
Crystal structure determination of biological macromolecules using the novel technique of
serial femtosecond crystallography (SFX) is severely limited by the scarcity of X-ray free …

A bright future for serial femtosecond crystallography with XFELs

LC Johansson, B Stauch, A Ishchenko… - Trends in biochemical …, 2017 - cell.com
X-ray free electron lasers (XFELs) have the potential to revolutionize macromolecular
structural biology due to the unique combination of spatial coherence, extreme peak …

Materials characterization by synchrotron x-ray microprobes and nanoprobes

L Mino, E Borfecchia, J Segura-Ruiz, C Giannini… - Reviews of Modern …, 2018 - APS
In recent years synchrotron x-ray microprobes and nanoprobes have emerged as key
characterization tools with a remarkable impact for different scientific fields including solid …

Human Adenosine A2A Receptor: Molecular Mechanism of Ligand Binding and Activation

B Carpenter, G Lebon - Frontiers in pharmacology, 2017 - frontiersin.org
Adenosine receptors (ARs) comprise the P1 class of purinergic receptors and belong to the
largest family of integral membrane proteins in the human genome, the G protein-coupled …

Recent advances in structure-based drug design targeting class AG protein-coupled receptors utilizing crystal structures and computational simulations

Y Lee, S Basith, S Choi - Journal of Medicinal Chemistry, 2018 - ACS Publications
G protein-coupled receptors (GPCRs) represent the largest and most physiologically
important integral membrane protein family, and these receptors respond to a wide variety of …

[PDF][PDF] Toward G protein-coupled receptor structure-based drug design using X-ray lasers

A Ishchenko, B Stauch, GW Han, A Batyuk, A Shiriaeva… - IUCrJ, 2019 - journals.iucr.org
Rational structure-based drug design (SBDD) relies on the availability of a large number of
co-crystal structures to map the ligand-binding pocket of the target protein and use this …