The interior lumen of acidic organelles (eg, endosomes, secretory granules, lysosomes and
plant vacuoles) is an important platform for modification, transport and degradation of …

Live and deep imaging play a significant role in the physiological and biological study of
organisms. Two-photon excitation microscopy (2PEM), also known as multiphoton excitation …

Metabolic reactions in living cells are limited by diffusion of reagents in the cytoplasm. Any
attempt to quantify the kinetics of biochemical reactions in the cytosol should be preceded by …

MCherry, the Discosoma sp. mushroom coral-derived monomeric red fluorescent protein
(RFP), is a commonly used genetically encoded fluorophore for live cell fluorescence …

The advent of easy-to-use open source microcontrollers, off-the-shelf electronics and
customizable manufacturing technologies has facilitated the development of inexpensive …

We present the application of multiphoton in vivo fluorescence correlation spectroscopy
(FCS) of fluorescent nanoparticles for the measurement of cerebral blood flow with excellent …

The ongoing co-circulation of multiple severe acute respiratory syndrome coronavirus 2
(SARS-CoV-2) strains necessitates advanced methods such as high-throughput multiplex …

Two cationic conjugated polyelectrolytes (CPEs) based on poly (phenylene ethynylene) are
found to exhibit efficient 2-photon excited fluorescence. In particular, the CPE aggregates …

Multiplex immunoassays with acellular antigens are well-established based on solid-phase
platforms such as the Luminex® technology. Cell barcoding by amine-reactive fluorescent …

mRNA–protein interactions play key roles in facilitating various biological functions in gene
expression regulations and even the progression of diseases. However, it is still a challenge …