Mass spectrometry-based techniques to elucidate the sugar code
Cells encode information in the sequence of biopolymers, such as nucleic acids, proteins,
and glycans. Although glycans are essential to all living organisms, surprisingly little is …
and glycans. Although glycans are essential to all living organisms, surprisingly little is …
Current Methods for the Characterization of O-Glycans
H Wilkinson, R Saldova - Journal of proteome research, 2020 - ACS Publications
Glycosylation is crucial in cellular metabolism and survival. Of interest is the role of N-linked
and O-linked glycans in disease states. Robust analytical methods must be defined to …
and O-linked glycans in disease states. Robust analytical methods must be defined to …
Butyrate producing colonic Clostridiales metabolise human milk oligosaccharides and cross feed on mucin via conserved pathways
MJ Pichler, C Yamada, B Shuoker… - Nature …, 2020 - nature.com
The early life human gut microbiota exerts life-long health effects on the host, but the
mechanisms underpinning its assembly remain elusive. Particularly, the early colonization of …
mechanisms underpinning its assembly remain elusive. Particularly, the early colonization of …
Structural analysis of N-and O-glycans released from glycoproteins
This protocol shows how to obtain a detailed glycan compositional and structural profile from
purified glycoproteins or protein mixtures, and it can be used to distinguish different isobaric …
purified glycoproteins or protein mixtures, and it can be used to distinguish different isobaric …
Glycan labeling strategies and their use in identification and quantification
Most methods for the analysis of oligosaccharides from biological sources require a glycan
derivatization step: glycans may be derivatized to introduce a chromophore or fluorophore …
derivatization step: glycans may be derivatized to introduce a chromophore or fluorophore …
High-sensitivity analytical approaches for the structural characterization of glycoproteins
1.1. General Considerations Structural intricacies of carbohydrate molecules and their
propensity to form varied linkages, substitutions, and branching patterns have fascinated …
propensity to form varied linkages, substitutions, and branching patterns have fascinated …
Glycan analysis by ion mobility–mass spectrometry
J Hofmann, K Pagel - Angewandte Chemie International …, 2017 - Wiley Online Library
Carbohydrates form one of the major classes of biological macromolecules in living
organisms. To investigate their properties and function, an in‐depth knowledge of their …
organisms. To investigate their properties and function, an in‐depth knowledge of their …
Antimicrobial defense systems in saliva
W van't Hof, ECI Veerman, AVN Amerongen… - Saliva: Secretion and …, 2014 - karger.com
The oral cavity is one of the most heavily colonized parts of our body. The warm, nutrient-rich
and moist environment promotes the growth of a diverse microflora. One of the factors …
and moist environment promotes the growth of a diverse microflora. One of the factors …
Structural glycomics using hydrophilic interaction chromatography (HILIC) with mass spectrometry
M Wuhrer, AR de Boer… - Mass spectrometry …, 2009 - Wiley Online Library
Hydrophilic interaction chromatography (HILIC) with mass spectrometry is a versatile
technique for structural glycomics. Glycans are retained by hydrogen bonding, ionic …
technique for structural glycomics. Glycans are retained by hydrogen bonding, ionic …