Mass spectrometry-based techniques to elucidate the sugar code

M Grabarics, M Lettow, C Kirschbaum, K Greis… - Chemical …, 2021 - ACS Publications
Cells encode information in the sequence of biopolymers, such as nucleic acids, proteins,
and glycans. Although glycans are essential to all living organisms, surprisingly little is …

Current Methods for the Characterization of O-Glycans

H Wilkinson, R Saldova - Journal of proteome research, 2020 - ACS Publications
Glycosylation is crucial in cellular metabolism and survival. Of interest is the role of N-linked
and O-linked glycans in disease states. Robust analytical methods must be defined to …

Butyrate producing colonic Clostridiales metabolise human milk oligosaccharides and cross feed on mucin via conserved pathways

MJ Pichler, C Yamada, B Shuoker… - Nature …, 2020 - nature.com
The early life human gut microbiota exerts life-long health effects on the host, but the
mechanisms underpinning its assembly remain elusive. Particularly, the early colonization of …

Structural analysis of N-and O-glycans released from glycoproteins

PH Jensen, NG Karlsson, D Kolarich, NH Packer - Nature protocols, 2012 - nature.com
This protocol shows how to obtain a detailed glycan compositional and structural profile from
purified glycoproteins or protein mixtures, and it can be used to distinguish different isobaric …

[PDF][PDF] Wang

Y Wang - Jia Z, Yi Q, Song L. The various components implied …, 2018 - tranassoc.com
Systems and methods are disclosed for communicating data in an integrated sensor network
having one or more nodes coupled to an optical sensor network and a radio frequency …

Glycan labeling strategies and their use in identification and quantification

LR Ruhaak, G Zauner, C Huhn, C Bruggink… - Analytical and …, 2010 - Springer
Most methods for the analysis of oligosaccharides from biological sources require a glycan
derivatization step: glycans may be derivatized to introduce a chromophore or fluorophore …

High-sensitivity analytical approaches for the structural characterization of glycoproteins

WR Alley Jr, BF Mann, MV Novotny - Chemical reviews, 2013 - ACS Publications
1.1. General Considerations Structural intricacies of carbohydrate molecules and their
propensity to form varied linkages, substitutions, and branching patterns have fascinated …

Glycan analysis by ion mobility–mass spectrometry

J Hofmann, K Pagel - Angewandte Chemie International …, 2017 - Wiley Online Library
Carbohydrates form one of the major classes of biological macromolecules in living
organisms. To investigate their properties and function, an in‐depth knowledge of their …

Antimicrobial defense systems in saliva

W van't Hof, ECI Veerman, AVN Amerongen… - Saliva: Secretion and …, 2014 - karger.com
The oral cavity is one of the most heavily colonized parts of our body. The warm, nutrient-rich
and moist environment promotes the growth of a diverse microflora. One of the factors …

Structural glycomics using hydrophilic interaction chromatography (HILIC) with mass spectrometry

M Wuhrer, AR de Boer… - Mass spectrometry …, 2009 - Wiley Online Library
Hydrophilic interaction chromatography (HILIC) with mass spectrometry is a versatile
technique for structural glycomics. Glycans are retained by hydrogen bonding, ionic …